. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SBTI-INDUCED POLYSPERMY 477. 2 4 6 8 SBTI (uM) 10 FIGURE 2. Effects of acid (D) or alkali (•) pretreatment on the ability of native (•) SBTI to cause polyspermy in Arbacia eggs as described under Methods. rs = Thus, there is a statistical correlation between enzyme inhibition and polyspermy promotion by these serine protease inhibitors. DISCUSSION The question of specificity is a major concern in all studies involving the use of inhibitors. Past evidence for specificity in the induction of polyspermy by SBTI rested TAB
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SBTI-INDUCED POLYSPERMY 477. 2 4 6 8 SBTI (uM) 10 FIGURE 2. Effects of acid (D) or alkali (•) pretreatment on the ability of native (•) SBTI to cause polyspermy in Arbacia eggs as described under Methods. rs = Thus, there is a statistical correlation between enzyme inhibition and polyspermy promotion by these serine protease inhibitors. DISCUSSION The question of specificity is a major concern in all studies involving the use of inhibitors. Past evidence for specificity in the induction of polyspermy by SBTI rested TABLE I Comparison of ID50 and Pi0for inhibition of egg protease and promotion of polyspermy by serine protease inhibitors Inhibitor ID50 (M) P50 (M) Soybean trypsin inhibitor X 1(T9 X 10~6 Ovomucoid X 10"6 X 10'4 Lima bean trypsin inhibitor X 10~6 X 10~4 Leupeptin X 10-6 X lO'4 Antipain x 10 5 X 10-4 ID50 = the inhibitor concentration at which 50% of the enzyme activity purified from S. purpuratus eggs is abolished as described under Methods using BAEE as substrate. P50 = the inhibitor concentration at which 50% polyspermy occurred in A. punctulata eggs, as described under Methods. The incidence of polyspermy for control eggs in these experiments was + (n = 15).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
