Archive image from page 12 of Design and operation of a. Design and operation of a carbon-14 biosynthesis chamber . designoperationo911smit Year: 1962 ±4 inches of water was created by a 3° F. tem- perature variation. A safety valve was designed to prevent greater fluctuations (fig. 10). The de- irice was composed of two vessels containing so- dium hydroxide solution to the level that pressure inside the chamber or outside would force air through the safety valve at a rate great enough to prevent dangerous pressure buildup. Air pass- ing through the trap was washed free of C02 and radioactivi
Archive image from page 12 of Design and operation of a. Design and operation of a carbon-14 biosynthesis chamber . designoperationo911smit Year: 1962 ±4 inches of water was created by a 3° F. tem- perature variation. A safety valve was designed to prevent greater fluctuations (fig. 10). The de- irice was composed of two vessels containing so- dium hydroxide solution to the level that pressure inside the chamber or outside would force air through the safety valve at a rate great enough to prevent dangerous pressure buildup. Air pass- ing through the trap was washed free of C02 and radioactivity. This prevented radioactive con- tamination from exhaust air and changes in spe- cific activity of C02 in the chamber by incoming air. This trap is rarely required to function. A manometer is located on the end of the cham- ber to indicate pressure differences between the inside and the outside of the chamber. OPERATION OF THE CHAMBER Soybeans, corn, wheat, and tobacco have been grown in the biosynthesis chamber. Although the same system of culture and environmental control was used for all crops, each crop presented differ- ent nutritional problems. With all the crops except tobacco the seeds were germinated between moist layers of muslin in a Pyrex tray on a stainless steel screen, then trans- ferred to nutrient solution for a few days for root and stem elongation. The soybean plants were then placed in the chamber supported in cork stop- pers with their roots in nutrient solution. The 3orn and wheat plants were supported in funnels filled with sand. These funnels were made from 200-ml. Erlenmeyer flasks from which the bot- toms had been removed. The necks of the funnels extended into the nutrient solution in the culture vessels. The basic nutrient used was one described by Steinberg (8) and modified by reducing the phos- phorus concentration to half. The normal con- centration of phosphorus was too high for grow- ing plants in the high C02 concentration of the chamber,
Size: 1710px × 1169px
Photo credit: © Actep Burstov / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: 1960, 1962, allison_franklin_e_franklin_elmer_1892_1972, archive, book, bookauthor, bookdecade, bookpublisher, booksponsor, booksubject, bookyear, carbon, drawing, growth_cabinets_and_rooms, growth_plants_, historical, history, illustration, image, mullins_joseph_f_1894_, page, picture, print, reference, smith_j_h_jay_hamilton_1927_, u_s_department_of_agriculture_national_agricultural_, united_states_department_of_agriculture, vintage, washington_d_c_agricultural_research_service_u_s_d
