Pediatrics. . es of mycelium from each branches form a dense interwoven trellis, which at the endof ten days reaches maturity and develops chlamydospores at theend of the filaments. The hanging drop can then be dried in the in-cubator, fixed with acetic acid in the flame, and stained as beforedescribed. When the chlamydospores are mature the mvceliumshrivels and ceases to take the stain (Fig. 6). On a three per cent, glucose agar, made with placenta bouillon,the growth has particularly long aerial filaments, which have asilky gloss and most geometrical plications. There is on
Pediatrics. . es of mycelium from each branches form a dense interwoven trellis, which at the endof ten days reaches maturity and develops chlamydospores at theend of the filaments. The hanging drop can then be dried in the in-cubator, fixed with acetic acid in the flame, and stained as beforedescribed. When the chlamydospores are mature the mvceliumshrivels and ceases to take the stain (Fig. 6). On a three per cent, glucose agar, made with placenta bouillon,the growth has particularly long aerial filaments, which have asilky gloss and most geometrical plications. There is only a faintdevelopment of the yellow color, due to the scarcity of identity of the fungus from the two sources is well shown Fig. V.—Microsporon felineum (X. aged 16). Culture on beer-wort agarfrom ringworm of the face (2 weeks growth). Description: Large,flat, circular downy growth. Central button in buff-colored area. Con-centric ring of buff color, with tangential fringe radiating to the Fig. VI.—Multilocular spindles or chlamydospores in hanging drop cultureof beer-wort. Inoculation made from ringworm of the cat. Spindlesare about 20 x 60 micra, and are divided into from five to eight com-partments. When the spindles are mature the mycelium bearing themdwindles and ceases to take the stain. RINGWORM 229 in cultures at the end of two weeks on placenta-glucose (three percent.) agar. Figs. 7 and 7a. This placenta-glucose agar is made in the following manner:A human placenta, weighing about five hundred grammes, ischopped fine and soaked over night in one liter of water. Squeezeout, boil, and skim. Add glucose 30, peptone 10, sea salt 5, boil,
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