. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SURFACE ANTIGENS IN KKA.\ examined for presence of a precipitate ring at the interface. All nine sera pro- duced rings of antigen-antibody complex at all antigen dilutions down through 1 :10,000. Controls were negative. The tests show, then, the presence of anti- bodies to sponge antigens extractible in saline, although these do not necessarily mean that cell surface antigens are involved. Tests of antiserum effects on reaggregation. Three ml. of an antiserum were mixed with an equal volume of double-strength van't I loff so
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SURFACE ANTIGENS IN KKA.\ examined for presence of a precipitate ring at the interface. All nine sera pro- duced rings of antigen-antibody complex at all antigen dilutions down through 1 :10,000. Controls were negative. The tests show, then, the presence of anti- bodies to sponge antigens extractible in saline, although these do not necessarily mean that cell surface antigens are involved. Tests of antiserum effects on reaggregation. Three ml. of an antiserum were mixed with an equal volume of double-strength van't I loff solution alpha ( grams NaCl, grams KC1, grams CaCl2, grams MgCl,.6HX>, grams , distilled water to one liter mark), producing a medium ap- proximately isosmotic with sea water. Four ml. of suspension 4, 5 or 6, freshly prepared, were added. Controls were 4-ml. portions of the same cell suspension added to three ml. of double-strength van't Hoff solution plus three ml. of serum from a normal uninjected rabbit (18 experiments), or to six ml. of ordinary strength van't Hoff (four experiments). In live experiments there were no simul- taneous controls. Altogether 63 antiserum and 22 control preparations were observed in 27 ex- periments; normal reaggregation was also followed in 13 other preparations, 10 in normal serum and three in van't Hoff solution. RESULTS Microciona in anti-Mierociona sent in Reaggregation curves for Microciona cells during the first three hours in normal serum and in two anti-Microciona sera are shown in Figure 3 with photomicro- graphs at hours. In two other antiserum preparations studied by reaggrega- tion curves, results were obtained scarcely distinguishable from the two pictured here. EFFECT OF ANTI-MICROCIONA SERUM ON MICROCIONA REAGGREGATION UJ Lu £0 <r u a. en 16 1 UJ o (T. ANTI-MCROGONA SERUM - I -- ANTI-MICROCIONA SERUM -2 NORMAL SERUM TIME IN HOURS FIGURE 3. Effect of anti-Microciona serum on Microciona
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
