. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 180 LYERLA, LYERLA AND FISHER • 80 g-60 2 CL b .40 Q O -20 ADULT PIGMENT EMBRYO PI&7MENT. 260 16 Q p •12 m 06 CP 3 O 2 O K m 04 350 450 550 1 nm FIGURE 1. Absorption spectra of purified mantle pigment in 50% methanol (left) and embryo pigment (right) in petroleum ether. O. D. scales have been adjusted to provide curves of similar heights. ing 70% dioxane, 50% methanol acidified to pH 3 with IN HC1, a mixture of 4 parts butanol: 1 part acetic acid: 1 part water and 10% trichloroacetic acid in 40% acetone (TCA-acetone). Ex
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 180 LYERLA, LYERLA AND FISHER • 80 g-60 2 CL b .40 Q O -20 ADULT PIGMENT EMBRYO PI&7MENT. 260 16 Q p •12 m 06 CP 3 O 2 O K m 04 350 450 550 1 nm FIGURE 1. Absorption spectra of purified mantle pigment in 50% methanol (left) and embryo pigment (right) in petroleum ether. O. D. scales have been adjusted to provide curves of similar heights. ing 70% dioxane, 50% methanol acidified to pH 3 with IN HC1, a mixture of 4 parts butanol: 1 part acetic acid: 1 part water and 10% trichloroacetic acid in 40% acetone (TCA-acetone). Extracts in these solvents provided clear yellow solutions after filtration that remained stable at room temperatures under normal lighting conditions. The TCA-acetone extracted pigment purified as described above and redissolved in 50% methanol migrated as a single fraction of Rf on cellulose thin-layer chromatograms using TCA-acetone as the developing solvent. The chromatograms showed no fluorescence, but the yellow pigment ab- sorbed either long or short wave UV irradiation. The addition of potassium borohydride to the TCA-acetone extracts to a con- centration of 1 M decolorized the solution, whereas the addition of 1 M sodium nitrite did not. The TCA-acetone extracted pigment could be dialyzed using TCA- acetone as the dialyzing medium but was retained as a flocculent brown precipitate when dialyzed against distilled water. The absorption spectrum of dialyzed pigment dissolved in 50% methanol is shown in Figure 1. The pigment absorbs strongly in the UV region, exhibiting a sharp peak at 360 nm. Absorbence in the visible range is general, shows no peaks, and falls to zero at wavelengths greater than 450 nm. Egg and embryo pigment was soluble in acetone, petroleum ether or hexane. The extracted pigment separated into three visible fractions on silica-gel TLC using a 1 hexane: 3 ethyl acetate mixture as developing solvent. The absorption spectrum in petroleum ether for
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
