. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B 2°Ab Pre-immune anti-coag FL MA F24 FL MA F24 FL MA F24 **. Figure 2. Protein gel and western blot analysis of total blood proteins from horseshoe crabs collected in Cape Cod (MA) and Tampa Bay (FL). and column fraction 24 (F24). (A) Coomassie-stained gel showing the three protein samples and molecular weight markers (kDa). The arrowhead to the left indicates the coagulogen protein band. (B) Blots from protein gels run in parallel with the gel shown in panel A. The blots were probed with the anti-coagulogen serum
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B 2°Ab Pre-immune anti-coag FL MA F24 FL MA F24 FL MA F24 **. Figure 2. Protein gel and western blot analysis of total blood proteins from horseshoe crabs collected in Cape Cod (MA) and Tampa Bay (FL). and column fraction 24 (F24). (A) Coomassie-stained gel showing the three protein samples and molecular weight markers (kDa). The arrowhead to the left indicates the coagulogen protein band. (B) Blots from protein gels run in parallel with the gel shown in panel A. The blots were probed with the anti-coagulogen serum (anti-coag), pre-immune serum, or with secondary antibody (2°Ab) alone. The arrowhead at the right indicates the position of coagulogen. The asterisk indicates a high molecular mass band in the MA sample that binds the secondary antibody. also observed blood cells in close association with isolated yolk masses (Fig. 3F, G). Discussion Amebocyte production beginx at a later stage in Limulus polyphemus than in Tachypleus tridentatus Using a combination of histological stains and an anti- body that specifically recognizes the coagulogen protein in amebocytes and on protein blots, we have shown that ame- bocyte production in L. polyphenius begins at stage 18 of development. Amebocytes were identified in all subsequent embryonic stages (stages 19-21, days 7-14 post-fertiliza- tion) and in the trilobite larvae, but not in embryos prior to stage 18. Stage 18 occurs during the 6th day post-fertilization and coincides with the first embryonic molt. At this stage the dorsal half of the embryo is still rounded and lacks distin- guishing marks. The ventral side of the embryo is flattened, the prosomal appendages (chelicerae, pedipalps, and walk- ing legs) have begun to lengthen, the stomodaeum is located slightly anterior to the chelicerae, and the lateral organs have begun to develop (Sekiguchi et ai. 1982). As men- tioned previously, Liang et nl. (1990) reported the identifi- cation o
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
