. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. Control of Oxygen Utilization 109 dicoumarol and ADP has been mentioned previously (Chance, 1956), and is repeated here (Fig. 14). Here we compare the absorption bands of the cytochromes which disappear upon addition of ADP with those which disappear upon addition of dicoumarol. If, for example, there were a selective effect of dicoumarol, then one would expect differences in the re- sponses to these agents. Treatment of the mitochondria with. ADP addition to state 4 Dicoumarol addition to state 4 - + 550 5
. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. Control of Oxygen Utilization 109 dicoumarol and ADP has been mentioned previously (Chance, 1956), and is repeated here (Fig. 14). Here we compare the absorption bands of the cytochromes which disappear upon addition of ADP with those which disappear upon addition of dicoumarol. If, for example, there were a selective effect of dicoumarol, then one would expect differences in the re- sponses to these agents. Treatment of the mitochondria with. ADP addition to state 4 Dicoumarol addition to state 4 - + 550 570 Q. o Fig. 14. Spectroscopic changes caused by addition of ADP, upper trace, compared with those caused by dicoumarol, lower trace. Both traces show an oxidation of cytochromes c and b. The data are plotted so that a decrease of absorption corresponds to an upward deflexion on the traces. Rat liver mitochondria, 400 [ ADP, 14 [jlm dicournarol, ^-hydroxy- butyrate as substrate. (Expt no 468d-6J (From Chance, 1956. Reproduced by permission of the Editors, Proc. Ill int. Congr. Biochem.) ADP causes absorption bands due to cytochromes c + c^ (551 m[x) and b (562 mpi) to disappear, as indicated by the double-humped peak in the difference spectrum. Addition of dicoumarol affects the absorption bands to the same relative extent. An interesting feature of Fig. 14 is that the absorbancy changes caused by dicoumarol are slightly larger than those caused by ADP, which appears to be inconsistent with the nearly identical respiratory stimulation caused by these two substances. In order to determine whether this is due to. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on the Regulation of Cell Metabolism (1958 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Ce
Size: 2182px × 1146px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionb, bookpublisherbostonlittlebrown
