. Cytological technique; the principles underlying routine methods. Histology -- Technique; Cytology -- Technique. 26 CYTOLOGICAL TECHNIQUE spaces filled with mounting medium coagulated ground cytoplasm. precipitate DMA Fig. 4. A typical spermatogonium, as seen in a routine micro- scopical preparation {diagrammatic). The cell has been fixed in a coagulant fixative, embedded in paraffin, dyed, and mounted. Compare with fig. 1 (p. 4). No traces of the mitochondria or lipid droplets remain. The centriole, if present, is intact; the idiozome sometimes but not always persists. The nuclear sap has b
. Cytological technique; the principles underlying routine methods. Histology -- Technique; Cytology -- Technique. 26 CYTOLOGICAL TECHNIQUE spaces filled with mounting medium coagulated ground cytoplasm. precipitate DMA Fig. 4. A typical spermatogonium, as seen in a routine micro- scopical preparation {diagrammatic). The cell has been fixed in a coagulant fixative, embedded in paraffin, dyed, and mounted. Compare with fig. 1 (p. 4). No traces of the mitochondria or lipid droplets remain. The centriole, if present, is intact; the idiozome sometimes but not always persists. The nuclear sap has been coagulated, like the ground cytoplasm. The heterochromatic segments of the chromo- somes are still visible and strongly dyed, but the rest of the DNA has been thrown down in the form of an irregular pre- cipitate scattered here and there on the strands of the coagulum and on the inside of the nuclear membrane. The nucleoli retain their form but are somewhat shrunken. The non-coagulant fixatives, especially osmium tetroxide and formaldehyde, cause much less initial change in structure than the coagulants. Indeed, while the cells still lie in these fixatives. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Baker, John Randal, 1900-. London, Methuen; New York, Wiley
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