. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. dUT DIG ^ PCR Reaction DIG labeledMarker :? Marker Dot Blot o o o o o o o o o o DIG labeledMarker 5 r^ Co^rrjjcl Di J A cJjJutJCjrj:. Figure 10. Digoxigenin Hybridization of Target STS marker to Cosmid DNA Dot Blot. The gray represents cosmid DNA which has bound the DIG labeled marker DNA. AGAROSE Gel Electrophoresis. Agarose gel electrophoresis was employed to determine the relationship among positive cosmids from hybridization and to analyze PCR results. Agarose gel electrophoresis is the standard
. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. dUT DIG ^ PCR Reaction DIG labeledMarker :? Marker Dot Blot o o o o o o o o o o DIG labeledMarker 5 r^ Co^rrjjcl Di J A cJjJutJCjrj:. Figure 10. Digoxigenin Hybridization of Target STS marker to Cosmid DNA Dot Blot. The gray represents cosmid DNA which has bound the DIG labeled marker DNA. AGAROSE Gel Electrophoresis. Agarose gel electrophoresis was employed to determine the relationship among positive cosmids from hybridization and to analyze PCR results. Agarose gel electrophoresis is the standard method of separating DNA of lengths from 200 base pairs to 50 kilobases for identification and purification procedures.^"^ A rectangular plate of agarose gel is subjected to a constant voltage and the DNA loaded into the gel. obtained by PCR or other means, migrates toward the positive electrode due to its negative charge (Figure 11 .a). When a gel is run with a DNA ladder of knovm size it is easy to determine the sizes of the DNA that are separated across the gel in the procedure (FIGURE 11 .b). By plotting the migration distances of known fragments as well as unknown fragments versus the sizes of the known fragments on a logarithmic plot one can 21. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Belk, Stephanie Heather.
Size: 2176px × 1148px
Photo credit: © Paul Fearn / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, booksubjectchromo, booksubjectsweetbriarcollege
