. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 152 PEZALLA, DORES AND HERMAN TABLE II Effects of Srphadex G-25 fractions of CNS extracts from Limulus in Orconectes and Uca. Fraction tested Uca response* Orconectes response** I II III Solvent controls ± (10) ± (10) db (10) (10) ± (10) ± (10) ± (10) ± (10) * Mean net chromatophore response; 10 n\ of pooled fraction injected. ** Percentage of increase in total carbohydrate 60 min after injection; 100 n\ of pooled fraction injected. about one-third in 1 hr. On
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 152 PEZALLA, DORES AND HERMAN TABLE II Effects of Srphadex G-25 fractions of CNS extracts from Limulus in Orconectes and Uca. Fraction tested Uca response* Orconectes response** I II III Solvent controls ± (10) ± (10) db (10) (10) ± (10) ± (10) ± (10) ± (10) * Mean net chromatophore response; 10 n\ of pooled fraction injected. ** Percentage of increase in total carbohydrate 60 min after injection; 100 n\ of pooled fraction injected. about one-third in 1 hr. On the basis of the above findings we now routinely boil the crude extracts briefly, centrifuge, and add thiodiglycol to a final concentration of 10"3 M. In addition, all extractions and chromatographic separations are per- formed at 4° C The next concern was to estimate the molecular weight of the LHGF via gel filtration. Initial separation of LHGF and LUC on Sephadex G-25 clearly demon- strated that a larger grade Sephadex was required; G-50 was selected. The results o CO CO co ^. UJ o r- Q_ o 0 8 04 Chymotrypsmogen ( 2 5,000) . Lysozyme (14,000). 0 I Kav / \. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
Size: 1319px × 1894px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
