. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. 258 F. Lynen, et al. (Lynen, 1941). It was found that the concentration of in- organic phosphate under oxygen was lower than under nitro- gen. If yeast cells are suspended in glucose solution and shaken alternatively under oxygen and nitrogen, the free phosphate oscillates between the lower aerobic and the higher anaerobic level (Fig. 2). However, the differences observed do not seem to be large enough to explain the considerable slowing down. Fig. 2. The orthophosphate content of baker's yeast, aerobically and
. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. 258 F. Lynen, et al. (Lynen, 1941). It was found that the concentration of in- organic phosphate under oxygen was lower than under nitro- gen. If yeast cells are suspended in glucose solution and shaken alternatively under oxygen and nitrogen, the free phosphate oscillates between the lower aerobic and the higher anaerobic level (Fig. 2). However, the differences observed do not seem to be large enough to explain the considerable slowing down. Fig. 2. The orthophosphate content of baker's yeast, aerobically and anaerobically. Yeast, 10 per cent; glucose, 6 per cent; under oxygen and nitrogen alternatively; temp. 30°. of triose phosphate dehydrogenase caused by respiration. In an attempt to overcome the difficulty, we considered the possibility of some of the inorganic phosphate in the cell, as determined by the molybdenum blue method, being present not as free phosphate, but as having existed as some labile phosphorus compound, acyl phosphate, which had become hydrolysed during assay (Lynen, 1941; Kamen and Spiegelman, 1948). More recent observations and the negative result of attempts to find noticeable amounts of acyl phosphate in respiring or fermenting yeast cells (Holzer, 1949) made it. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on the Regulation of Cell Metabolism (1958 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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