. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IMAGING MEMBRANE POTENTIAL PHO100IOOE OUTPUTS 114 114 3 3. NEURON ACTIVITY S Figure 4. Optical recordings from a portion of a photodiode array from an A/j/w/a abdominal ganglion. The drawing to the left represents the relative position of the detectors whose activity is displayed. In the top right, the original data from seven detectors are illustrated. The numbers to the right of each trace identify the detector from which the trace was taken. The bottom section shows the raster diagram illustrating the results of our s
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IMAGING MEMBRANE POTENTIAL PHO100IOOE OUTPUTS 114 114 3 3. NEURON ACTIVITY S Figure 4. Optical recordings from a portion of a photodiode array from an A/j/w/a abdominal ganglion. The drawing to the left represents the relative position of the detectors whose activity is displayed. In the top right, the original data from seven detectors are illustrated. The numbers to the right of each trace identify the detector from which the trace was taken. The bottom section shows the raster diagram illustrating the results of our sorting of these data into the spike activity of four neurons. At the number 1 in the top section there are synchronously occurring spike signals on all seven detectors. A synchronous event of this kind occurs more than 20 times; we presume that each event represents an action potential in one relatively large neuron. The activity of this cell is represented by the vertical lines on trace 1 of the bottom section. The activity of a second cell is indicated by small signals at the number 4 on 119 and its neighbor. 124. The activity of this cell is represented by the vertical lines on trace 4 of the bottom section. The activity of neurons 2 and 3 was similarly identified. (Modified from Zecevic el al. 1989.) of the command pulse to the solenoid controlling odor delivery. We optimized the optics for measurements of epifluores- cence at low magnification. In this circumstance the inten- sity reaching the objective image plane is proportional to the fourth power of the numerical aperture (NA) of the objec- tive (Inoue, 1986). Because conventional microscope optics have small numerical apertures at low magnifications, we assembled a microscope (macroscope) based on a 25-mm focal length, f, C-mount, camera lens (used with the C-mount end facing the preparation) (Salama, 1988: Ratzlaff and Grinvald, 1991; Kleinfeld and Delaney. 1996). With a magnification of 4X, the intensity reachi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
