. Carnegie Institution of Washington publication. 35 45 t n. r n. f n. r n. 1 1 —I2 -1 Sj 6 R 9 1 ^^ ^ \ ^. p. / 3 B | 1 1 1 7 FIG. 12. Continuation of record of variations of sec- tions of agar and bean protein in fig. 11. For description see text, pages 81 and 82. m. 35 45. m. jn. 12 I New sheets were fitted to the recorder of the auxograph and arrange- ments made to follow further changes (see fig. 12). During the next 4 days an additional swelling of 280 per cent in the malic series, 320 per cent in the citric, and 300 per cent in the f
. Carnegie Institution of Washington publication. 35 45 t n. r n. f n. r n. 1 1 —I2 -1 Sj 6 R 9 1 ^^ ^ \ ^. p. / 3 B | 1 1 1 7 FIG. 12. Continuation of record of variations of sec- tions of agar and bean protein in fig. 11. For description see text, pages 81 and 82. m. 35 45. m. jn. 12 I New sheets were fitted to the recorder of the auxograph and arrange- ments made to follow further changes (see fig. 12). During the next 4 days an additional swelling of 280 per cent in the malic series, 320 per cent in the citric, and 300 per cent in the formic were recorded. Replacement of the acid by hydroxid (1) resulted first in an expan- sion which was partially lost in 7 hours, so that the net gain was very light. When the hydroxid was washed off (2) hydration in distilled water was followed by expansion of lesser amplitude than in the previous procedure of this kind, but it had not ceased at the end of 14 hours. A diminution in each repetition was found. Hydroxid (3) failed to bring the sections back to the dimensions preceding the last hydration. Replacement of the hydroxid by acid (4) caused a further slight contraction, but not to the last pre-hydration dimen- sions. In fact, every hydration included an irreversible element. Hydroxid (5) again produced shrinkage, and then contraction which soon ceased. After 13 hours in hydroxid, water applied and re- newed (6) produced a swelling which was in progress at the end of 12 hours. Replacement with acids (7) was followed by very abrupt shrinkages which were more gradual in formic acid. Substitution of hydroxid after 11 hours at 8 was followed by the expected initial expansion and subsequent shrinkage. The final hydration (9) on the tenth day of the test gave swellings with net expansions of 7, 9, and 8, as compared with 21, 18, and 17 on the sixth day. The biocolloid is thus seen to progress through a period of reactions of increasing amplitude to a climax, followed by one
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