. Biological structure and function; proceedings. Biochemistry; Cytology. 82 J, B. CHAPPELL presence of io~^ m DNP only glutamate and isocitrate oxidation occur at these rates; both malate (600-800 fxl. Oo/mg. N/hr.) and succinate (up to 1600 ^1. Oo/mg. N/hr.) oxidation occur at considerably greater rates. These results indicate that many mitochondrial oxidations are controlled by the activity of the enzymes directly involved in the synthesis of ATP and subsequent to the site of action of DNP. On the other hand ^-hydroxy- butyrate was oxidized at only 60'^,, of the ADP-rate for other DPN Outsi
. Biological structure and function; proceedings. Biochemistry; Cytology. 82 J, B. CHAPPELL presence of io~^ m DNP only glutamate and isocitrate oxidation occur at these rates; both malate (600-800 fxl. Oo/mg. N/hr.) and succinate (up to 1600 ^1. Oo/mg. N/hr.) oxidation occur at considerably greater rates. These results indicate that many mitochondrial oxidations are controlled by the activity of the enzymes directly involved in the synthesis of ATP and subsequent to the site of action of DNP. On the other hand ^-hydroxy- butyrate was oxidized at only 60'^,, of the ADP-rate for other DPN Outside. Inside /socitrate dehydrogenase Oxaloacetate Oxaloacetate |-«-- DPN, arsenate Pyruvate Phosphoenol- pyruvate Inhibition -•- Fig. II. Postulated spatial relationship of some mitochondrial enz^Tnes. linked substrates; in this case some other factor, presumably the activity of the dehydrogenase, controls the rate of oxidation. Acknowledgment I wish to thank Miss Freda Johnson for her expert technical assistance. References 1. Chappell, J. B. (ig6i). To be published. 2. Chance, B., and Williams, G. R., Advanc. Enzy7noL l.' 65 (1956). 3. Purvis, J. L., BiocJu'm. hiopliys. Acta 30, 440 (1958). 4. Stein, A. M., Kaplan, N. O., and Ciotti, M. M.,y. biol. Chem. 234, 979 (1959). 5. Thorne, C. J. R., personal communication (i960). 6. Borst, P., and Slater, E. C, Biochim. hiophys. Acta 41, 170 (i960). 7. Krebs, H. A., and Bellamy, D., 75, 523 (i960). 8. Lardy, H. \., and Wellman, H.,7- biol. Chem. 195, 215 (1952).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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