. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AB. FIGURE 1. Overhead views of systems for determining the rate of sea water transport by the tunicate using the transfer of dyed sea water solution from compartment to compartment. The schematically rendered tunicate in the diagram shows the position of the attached specimen with respect to each compartment, dona which were used for experimental purposes lay horizontally, supported by the bottom of the container. Left shows dona attached by both siphons to Pyrex tubes with tygon tubing. Compartment A is attached to incurre
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AB. FIGURE 1. Overhead views of systems for determining the rate of sea water transport by the tunicate using the transfer of dyed sea water solution from compartment to compartment. The schematically rendered tunicate in the diagram shows the position of the attached specimen with respect to each compartment, dona which were used for experimental purposes lay horizontally, supported by the bottom of the container. Left shows dona attached by both siphons to Pyrex tubes with tygon tubing. Compartment A is attached to incurrent siphon; compartment B is attached to excurrent siphon; compartment C contains the body; tube D maintains equal levels. Right shows Ciona attached by incurrent siphon only to compartment AB. Compartment C contains the body. Plume of dye emitted by tunicate is schematically rendered. Return tube prevents the mixing of AB and C. Dunaliclla euchlora was grown at room temperature under fluorescent light banks in one gallon bottles in supplemented sea water type f (Guillard and Ryther, 1962). Stock cultures were similarly maintained sterilely at 19° C in 125 ml Erlen- meyer flasks. Algae were concentrated by centrifugation at 2500 rpm and resus- pended in fresb sea water. METHODS AND RESULTS Volume methods Specimens were attached to a partitioned chamber (Fie. 1, left, with con- necting tube D absent). A typical rate of transport for a g wet weight specimen was 180 ml hr'1 (or 45 ml/hr/g wet weight) determined by measuring the volume of overflow from Chamber B in 30 minutes. Careful observation showed that even under these conditions surface tension effects generated a pressure head approxi- mately 1 mm between Chambers A and Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
