. Cytology. Cytology. There are three methods that are commonly used to make contact between the specimen and the photographic emulsion. The first is the so-called opposition method, where the specimen is simply placed in contact with the photographic emulsion in the dark. This method is suitable for making autoradiographs of gross specimens or single cells. Paraffin sections of tissue can be floated on warm water and then picked up on a microscope slide which has previously been coated with a thin emulsion film. The sections remain attached to the slide during photo- RadioQCtive material. Pho
. Cytology. Cytology. There are three methods that are commonly used to make contact between the specimen and the photographic emulsion. The first is the so-called opposition method, where the specimen is simply placed in contact with the photographic emulsion in the dark. This method is suitable for making autoradiographs of gross specimens or single cells. Paraffin sections of tissue can be floated on warm water and then picked up on a microscope slide which has previously been coated with a thin emulsion film. The sections remain attached to the slide during photo- RadioQCtive material. Photographic emulsion Figure 11-36. Schematic Diagram Showing the Principle of Autora- diography. (From Pelc, S. R., 1958. "Autoradiography as a Cytochemical Method, with Special Reference to C^^ and S-^^," in Danielli, J. F. (Ed.), "General Cytochemical Methods," 1, Academic Press, New York, N. Y., Fig. l,p. 280.) graphic processing and subsequent staining and mounting. The second is the liquid-emulsion method which involves mounting the specimen on a slide and then coating it with a fluid emulsion that is allowed to harden. Extraction, hydrolysis, or staining of the specimen is generally carried out before application of the emulsion. The third method is the stripping- film technique which has been used extensively in this country and abroad. In this method, a thin-film emulsion is stripped from a glass plate and floated, emulsion side down, on the surface of water. The emulsion film is then picked up with a slide on which the specimen (parafiin sections, squashes) has been mounted. The specimen may be stained before or after it has been covered with the stripping film. 258 / CHAPTER 11. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wilson, G. B. (George Bernard), 1914-; Morrison, John H
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