. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 80 40 "c a> u Lkl M O e HI * 15 LU ft, ni n li 20 0. "0 10 20 30 40 50 60 010 20 30 40 50 60 Retention Time (min) Figure 7. Reverse phase-HPLC chromatograms of extracts of tissues from juvenile stage C4 lobsters. Hemolymph (a,h), abdominal muscle (). and midgut gland () were taken at either I h () or 10 days (b,d,f) after injection with ['H]-ecdysone. Samples were separated using gradient #3. Abbreviations for the various ecdysteroids are as in Figure 4. approximately equivalent amounts of 20E, ecdyso
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 80 40 "c a> u Lkl M O e HI * 15 LU ft, ni n li 20 0. "0 10 20 30 40 50 60 010 20 30 40 50 60 Retention Time (min) Figure 7. Reverse phase-HPLC chromatograms of extracts of tissues from juvenile stage C4 lobsters. Hemolymph (a,h), abdominal muscle (). and midgut gland () were taken at either I h () or 10 days (b,d,f) after injection with ['H]-ecdysone. Samples were separated using gradient #3. Abbreviations for the various ecdysteroids are as in Figure 4. approximately equivalent amounts of 20E, ecdysone, and apolar products were present in midgut gland at 1 h after injection. Smaller amounts of HP2, and a peak eluting between P and T, were also found in the midgut gland at 1 h. By 10 days, hemolymph contained mostly HP with a large peak of 20,26E and smaller quantities of HP1, 20EA, HP2, and 20E. In contrast, a large peak of HP1 and very small amounts of 20EA, , and 20E were present in the muscle at 10 days. A large amount of apolar material and smaller quantities of HP1, 20EA, , 20E. and ecdysone were found in the midgut gland at 10 days. Discussion At all molt stages, ecdysone was very rapidly eliminated from lobster hemolymph. The rapid loss of [3H]-ecdysone was not unexpected, because ecdysone tilers in H. amer- icanm (as determined by RIA) were never more than 19% of the total ecdysteroids in hemolymph (Snyder and Chang, 199la). Ecdysone also has a short half-life in the crab Gecarcinus lalcralis (McCarthy, 1980, 1982) and in several insect species (reviewed by Koolman and Karlson, 1985; Koolman and Walter, 1985). The activity of ec- dysone 20-monooxygenase, which converts ecdysone to 20-hydroxyecdysone (20E), probably has a role in chang- ing the levels of ecdysone in lobsters, as it does in several insects (Smith et ai. 1983: Mitchell and Smith, 1988). Young (1976) treated blowflies with supraphysiological doses of ecdysone, and demonstrated that alterati
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
