. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ASCIDIAN XENO- AND ALLO-REACTIVITV 307. FIGURE 2. Contact reaction between vesicular cells (v1 and v2) from two individuals of Type C H. rorctzi. One cell, v1 was vitally stained with Nile Blue and appears darker than the second, unstained, v2 cell. Following contact (a), both cells remain stationary (b), followed by lysis of v2 (v2', in c) and then v1 cell (v1' in d). Scale bar — 10 /*. Time elapsed after the cell contact is indicated in seconds at the lower left corner of each frame. Xenogeneic contact reactions were obser
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ASCIDIAN XENO- AND ALLO-REACTIVITV 307. FIGURE 2. Contact reaction between vesicular cells (v1 and v2) from two individuals of Type C H. rorctzi. One cell, v1 was vitally stained with Nile Blue and appears darker than the second, unstained, v2 cell. Following contact (a), both cells remain stationary (b), followed by lysis of v2 (v2', in c) and then v1 cell (v1' in d). Scale bar — 10 /*. Time elapsed after the cell contact is indicated in seconds at the lower left corner of each frame. Xenogeneic contact reactions were observed in congeneric species pairs as well as between species of different genera, for example, H. roretzi vs. H. aurantium, H. roretzi vs. P. Jiiirabilis, H. rorctzi vs. S. clava, P. niirabilis vs. S. clava, and C. intestinalis vs. C. robnsta. The strength of contact reactions in various species pairs, judged by the frequency of reactions and the average time required from cell contact to lysis, was roughly the same except possibly for reactions involving the two Cioua- species, whose reaction seemed somewhat weaker than that between other xenogeneic combinations examined. Three variants of H. roretzi, designated as Types A, B, and C, have been described by Numakunai and Hoshino (1973). They differ in peak daily spawn- ing hours, reproductive season, and preferred habitat, as well as in external morphology. Cells from different variants, when tested by mixing in vitro, exhibited vigorous contact reactions in all combinations of the three types. Although these three variants are taxonomically considered conspecific, genetic differences between any two types are probably intermediate between xenogeneic and allogeneic. It was therefore of interest to test whether cells from different individuals within the same variant could react with each other. In such experi- ments, contact reactions were observed in most but not all pairs, as discussed below. When an allogeneic contact reacti
Size: 3379px × 739px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
