. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PHYSIOLOGICAL EFFECTS OF HYDROGEN CYANIDE. 371 other body movements had ceased and that the organism did not recover after the heart 20 FIG. 4. Curve showing the effect of pH on the toxicity of HCN to Daphnia. Abscissae represents pH; ordinates time in minutes required to kill 95 per cent, of the organisms. It is obvious from Fig. 3 and 4 that less time was necessary to kill the animals in acid solution than in the alkaline solution. It required twenty-two minutes to kill Daphnia at a pH of to ; forty minute
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PHYSIOLOGICAL EFFECTS OF HYDROGEN CYANIDE. 371 other body movements had ceased and that the organism did not recover after the heart 20 FIG. 4. Curve showing the effect of pH on the toxicity of HCN to Daphnia. Abscissae represents pH; ordinates time in minutes required to kill 95 per cent, of the organisms. It is obvious from Fig. 3 and 4 that less time was necessary to kill the animals in acid solution than in the alkaline solution. It required twenty-two minutes to kill Daphnia at a pH of to ; forty minutes at a pH of and sixty-four minutes at a pH of The concentration of cyanide used in the above experiment was M/45O. The results indicated what would be expected from the study of the frog skin " cell," where more cyanide entered when the external pH was acid than when alkaline. The animals were killed first in solutions of the same pH values in which HCN penetrated the frog skin cells most quickly. The effect of the pH on penetration of cyanide was further checked by studying its effect on the streaming of protoplasm in Elodea cells (in press). The streaming of the protoplasm can be observed under the high power of a microscope while the cells are immersed in a solution of cyanide. The pH was Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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