. Annals of applied biology. Biology, Economic; Biochemistry. S. G. Paink 211 agar, the sixth transfer from the original, incubated at 22° C. The colony had attained a diameter of 4 mm. The fihn was fixed by ten minutes' immersion in 4 per cent, formah'n, stained for ten minutes in aqueous methyl violet, and examined in oil. The length of the organism varied from 0-9/x to 1-7/i and the breadth from 0-4/x to 0-5/x. The growth was very viscous and suggested the presence of a capsule; when a suspension of the organism in normal saline was examined by dark- ground illumination, however, no sign of
. Annals of applied biology. Biology, Economic; Biochemistry. S. G. Paink 211 agar, the sixth transfer from the original, incubated at 22° C. The colony had attained a diameter of 4 mm. The fihn was fixed by ten minutes' immersion in 4 per cent, formah'n, stained for ten minutes in aqueous methyl violet, and examined in oil. The length of the organism varied from 0-9/x to 1-7/i and the breadth from 0-4/x to 0-5/x. The growth was very viscous and suggested the presence of a capsule; when a suspension of the organism in normal saline was examined by dark- ground illumination, however, no sign of a capsule was discovered. Motility and Flagella. The organism is actively motile in young cultures in broth and upon agar, but it comes to rest early, frequently after 24 hours at air temperature on solid media. The movement is of a free swimming type without other specific characteristic. The flagella have been stained by Stevens' modification of Van Ermengen's stain and by a new method devised by the late Professor H. G. Plimmer^. The flagella are two to four times the length of the organism, one or two in number, occasionally three and more rarely four or five, arranged at one pole. (See Fig. 5.) The organism is therefore a ^ I \ Fig. 5. Camera drawing from a preparation stained by the method of Plimmer. Staining. The organism stains well with carbol fuchsin, gentian violet and methyl violet, does not stain by Gram's method, is not acid fast, and gives negative results with the usual capsule stains. ^ I hope with Dr R. H. A. PHmmer's permission to publish a description of this staining method if the details can be worked out from the data left by Professor Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Association of Applied Biologists; Association of Economic Biologists. [Welle
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbiochem, bookyear1919
