. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1 Sodeik, B., M. W. Ebersold, and A. Helenius. 1997. J. Cell Biol. 136: 1007-1021. 4. Vale, R. D., T. S. Reese, and M. P. Sheetz. 1985. Cell 42: 39-50. 5 Kuznetsov, S. A., G. M. Langford, and D. G. Weiss. 1992. Nature 356: 722-725. 6. Bearer E. L., J. A. DeGiorgis, R. A. Bodner, A. W. Kao, and T. S. Reese. 1993. Proe. Null. Acad. Sci. USA 90: 11252-11256. 7. Langford, G. M., S. A. Kuznetsov, D. Johnson, D. L. Cohen, and D. G. Weiss. 1994. J. Cell Sci. 107: 2291-8. 8 Bearer, E. L., J. A. DeGiorgis, N. A. Medeiros, and T. S. R
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 1 Sodeik, B., M. W. Ebersold, and A. Helenius. 1997. J. Cell Biol. 136: 1007-1021. 4. Vale, R. D., T. S. Reese, and M. P. Sheetz. 1985. Cell 42: 39-50. 5 Kuznetsov, S. A., G. M. Langford, and D. G. Weiss. 1992. Nature 356: 722-725. 6. Bearer E. L., J. A. DeGiorgis, R. A. Bodner, A. W. Kao, and T. S. Reese. 1993. Proe. Null. Acad. Sci. USA 90: 11252-11256. 7. Langford, G. M., S. A. Kuznetsov, D. Johnson, D. L. Cohen, and D. G. Weiss. 1994. J. Cell Sci. 107: 2291-8. 8 Bearer, E. L., J. A. DeGiorgis, N. A. Medeiros, and T. S. Reese. 1995. Cell Mori/. Cytoxkeleton 33: 106-114. 9. Schnapp, B. J., R. D. Vale, M. P. Sheetz, and T. S. Reese. 1985. Cell 40: 455-462. 10. Bearer, E. L., and T. S. Reese. 1999. J. Neurocytol. 28: 85-98. 11 Galbraith, J. A., T. S. Reese, M. L. Schlief, and P. E. Gallant. 1999. Proc. Natl. Acad. Sci. USA (in press). Figure 1. Retrograde transport of a GFP-labe/ed viral particle in u living squid axon. The giant axon was dissected in Ca++-containing seawater and injected with >IOO pfu O/GFP-VP16 labeled and extracted Herpes simplex virus. Still frames were taken from a BioRad confocal microscope sei/uence captured at 2—3 sec intervals and processed with NIH Image sojhvare. The diagram shows the orientation of the giant axon and site of injection relative to obsen-ed movements (dashed arrow). In this preparation, the cell body is to ihc right. GFP-labeled particle (oblii/ue arrowhead] moves at fiin/s towards the cell body, contrasting with a \tati<mar\ fluorescent spot (vertical arrow). Axons were dissected away from cell bodies and s\napses, and their two cuds labeled with color coding string. Bar = 10 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, M
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