. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. URINE-FORMATION IN CRAYFISH KIDNEY 249 cc. of the freshly prepared diphenylamine reagent. The tubes were capped and put into a boiling water bath for six minutes. The color which developed at the end of this time was evidently maximal. Un- aided visual examination of the intensity of color did not indicate any differences in the amount of inulin present in the tubes. The intensity was determined solely by the mass of tissue used. Regardless of whether a substance is removed from the blood by extrarenal tissues, the renal cle
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. URINE-FORMATION IN CRAYFISH KIDNEY 249 cc. of the freshly prepared diphenylamine reagent. The tubes were capped and put into a boiling water bath for six minutes. The color which developed at the end of this time was evidently maximal. Un- aided visual examination of the intensity of color did not indicate any differences in the amount of inulin present in the tubes. The intensity was determined solely by the mass of tissue used. Regardless of whether a substance is removed from the blood by extrarenal tissues, the renal clearance of the substance will be a function of the concentration of that substance in the blood. It was nevertheless of interest to find if inulin can be hydrolyzed by the tissues of the cray- - o o o o o o. o o FIG. 7. The U/P ratio of inulin, xylose, and creatinine (ordinate) as a func- tion of the concentration of these compounds in the plasma (abscissa). Notations identical with those in Fig. 6. Each point stands for a single separate animal; the same as those in Fig. 6. fish. The kidneys and samples of the hepatopancreas, somatic muscles, and blood were frozen in solid carbon dioxide, thoroughly macerated, and extracted in a known quantity of saline. To aliquots of the cen- trifugates were added a solution of inulin and a small drop of xylol. The mixtures were analyzed for inulin immediately and after 13 hours at room temperature. The controls contained only a solution of inulin and the preservative. There was no change in the concentration of reducing carbohydrate, with or without acid-hydrolysis, in any tube. This indicates that, under the conditions of the experiments at least, inulin is not hydrolyzed by the tissues of the crayfish. Similar experi- ments showed a destruction of d-xylose in the following descending. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of the
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
