. The microscope; an introduction to microscopic methods and to histology. Microscopes; 1899. 156 ISOLATION OF ELEMENTS. [CH. VII. eessful (§ 3121. The fresh muscle is placed in this in a glass vessel. At the ordinary temperature of a sitting room ('20 degrees centigrade) the connective tissue will be so far gelatinized in from one to three days that it is very easy to separate the fascicles and fibers either with nee- dles or by shaking in a test tube or reagent vial ( Fig. 132 ) with water. It takes longer for some muscles to dissociate than others, even in the same temperature, so one must
. The microscope; an introduction to microscopic methods and to histology. Microscopes; 1899. 156 ISOLATION OF ELEMENTS. [CH. VII. eessful (§ 3121. The fresh muscle is placed in this in a glass vessel. At the ordinary temperature of a sitting room ('20 degrees centigrade) the connective tissue will be so far gelatinized in from one to three days that it is very easy to separate the fascicles and fibers either with nee- dles or by shaking in a test tube or reagent vial ( Fig. 132 ) with water. It takes longer for some muscles to dissociate than others, even in the same temperature, so one must try occasionally to see if the action is sufficient. When it is, the acid is poured off and the muscle washed. Fig. 131. Adjustable lens holder for the same purposes as Fig. 130. {The Bausch & Lomb Optical Company). gently with water to remove the acid. If one is ready to make the prep- arations at once they may be isolated and mounted in water. If it is de- sired to keep the specimen indefinitely, or several days, the water should be poured off and a half saturated solution of alum added ( § 299). The alum solution is also very advantageous if the specimens are to be stained. The specimens may be mounted in glycerin, glycerin jelly or balsam. Glycerin jelly is the most satisfactory, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Gage, Simon Henry, 1851-1944. Ithaca, N. Y. , Comstock Publishing Company
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