. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. ORDINARY STAINING-SOLVTIONS 165 these simple. solutions it is necessary to employ agents that will increase the penetrating action of the dyes. Ex- perience has taught us that this can be accomplished by the addition to the solutions of small quantities of alkaline substances, or by dissolving the staining-materials in strong watery solutions of either aniline or carbolic acid, instead of water—in other words, by employing special solvents and mordants with the stains. Fig. 33. Rack of bottles for s
. The principles of bacteriology; a practical manual for students and physicians. Bacteriology. ORDINARY STAINING-SOLVTIONS 165 these simple. solutions it is necessary to employ agents that will increase the penetrating action of the dyes. Ex- perience has taught us that this can be accomplished by the addition to the solutions of small quantities of alkaline substances, or by dissolving the staining-materials in strong watery solutions of either aniline or carbolic acid, instead of water—in other words, by employing special solvents and mordants with the stains. Fig. 33. Rack of bottles for staining-solutibns. Of the solutions thus prepared which may always be employed upon bacteria that show a tendency to stain imperfectly, there are three in common use—^LofHer's alkaline methylene-blue solution; the Koch-Ehrlich ani- line-water solution of either fuchsin, gentian-violet, or methylene-blue; and Ziehl's solution of fuchsin in carbolic acid. These solutions are as follows: Ldffier's alkaline methylene-blue solution: Concentrated alcoholic solution of methylene-blue Caustic potash in 1: 10,000 solution . 30 100 Koch-Ehrlich aniline water solution. To about 100 of distilled water aniline oil is slowly added, a few drops. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Abbott, Alexander Crever, 1860-1935. Philadelphia, Lea & Febiger
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