Wakker's hyacinth germ, Pseudomonas hyacinthi (Wakker) . the moderate productionof alkali. In the presence of air produces an organic acid (probablyacetic) from ethyl alcohol dissolved in milk or bouillon. Inverts canesugar, but apparently without the intervention of any enzym. Will notgrow on 30 per cent grape-sugar agar. Resists dry air verj well, i. e.,more than forty-eight days when spread on cover glasses in thin Dunhams solution with metliylene blue the color is reduced ina few days, but reoxidizes quickly on shaking; final color (56 dajs)bright blue. In Dunhams solution with i
Wakker's hyacinth germ, Pseudomonas hyacinthi (Wakker) . the moderate productionof alkali. In the presence of air produces an organic acid (probablyacetic) from ethyl alcohol dissolved in milk or bouillon. Inverts canesugar, but apparently without the intervention of any enzym. Will notgrow on 30 per cent grape-sugar agar. Resists dry air verj well, i. e.,more than forty-eight days when spread on cover glasses in thin Dunhams solution with metliylene blue the color is reduced ina few days, but reoxidizes quickly on shaking; final color (56 dajs)bright blue. In Dunhams solution with indigo carmine the colorchanges to a bright blue, which persists for a long time; final coloryellowish. In Dunhams solution with rosolic acid and enough HCl. Fig. o.—Typical behavior of in fermentationtubes containing peptonewater, or peptonized beefbouillon, with addition of vari-ous sugars and other carbohy-drates. Fluid clear in closedend, clouded in U and openend. 42 to render the fluid yellowish, Ps. hyacintlii did not redden the fluid, butmade it colorless, the bacterial precipitate becoming rosy or salmon-colored. Produces indol slowly in peptonized beef broth and in pep-tonized Uschinskys solution; does not produce nitrites in thesesolutions. Does not reduce potassium nitrate to nitrite in peptonizedbeef bouillon. Not a strong-smelling germ. Not readily destro^^edbj^ its own decomposition products except in media containing not grow in the thermostat at 37° C, and grows very feebly onsome media and not at all on others at 34° to 35° C. Optimum tem-perature 28° to 30° C., or thereabouts. Minimum temperature approx-imately 4° C. Thermal death point (10 minutes exposure) °C.; nearly all the rods
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