Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . lar .ind «lets of fat that have born blackened by the i« Ov rhc rop- 244 GENERAL PATHOLOGY. Fatty degeneration affects particularly epithelial surfaces and glan-dular viscera, muscles, nerves, and blood-vessels; occasionally it is ob-served in the brain, and there constitutes a variety of softening. Incertain localities the process is apparently physiologic: for example, theproduction of fat by degeneration of the central cells of the acini, in themammary gland, leading to
Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . lar .ind «lets of fat that have born blackened by the i« Ov rhc rop- 244 GENERAL PATHOLOGY. Fatty degeneration affects particularly epithelial surfaces and glan-dular viscera, muscles, nerves, and blood-vessels; occasionally it is ob-served in the brain, and there constitutes a variety of softening. Incertain localities the process is apparently physiologic: for example, theproduction of fat by degeneration of the central cells of the acini, in themammary gland, leading to the formation of milk, and the fatty changeseen in the uterus after labor. (See Fatty Degeneration of the Heart,Blood-vessels, Liver, Part VII.) Deiiwiistratiou.—The highly refractive oil globules can usually berecognized under the microscope. In fresh tissues the affected cellsare not cleared up by acetic acid; they are blackened by osmic acid; theblack or brownish-black granules produced by treating fat with osmicacid are not soluble in alcohol during the subsequent dehydration, but y»*ij» m. Fig. 142.—Heart, Transverse and Oblique Sections of Muscle-fibers, showing Fatty preparation, from case of pernicious anemia. may undergo partial solution during the process of clearing for par-affin infiltration, and particularly when subjected to the action of fatsolvents, such as chloroform, turpentine, xylol, etc. For the demon-stration in sections fixation in alcohol is not applicable. Tissuesshould be fixed in one of the osmic acid solutions (see pp. 7,7, and 34),dehydrated, infiltrated with celloidin, and sectioned. The fat globuleswill be blackened by this method. Blocks of tissue may be fixed inMiillers fluid (see p. 31) or in Orths fluid (see p. 31) or in a ten percent, solution of formaldehyd for a week or ten days. The tissue isthen transferred to Marchis fluid, which consists of: Mtillers fluid, 2 parts. One per cent, aqueous solution of osmi
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