. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 204 BACTERIOLOGY perature. The growth quickly extends, but ceases after the lapse of only a few days. Experiments have been carried on by Wertheim at the clinic of Professor P. Schauta in Vienna, on the cultivation of the gonococcus upon a medium consisting of a mixture of two or three parts peptone glycerine agar and one part human blood-serum. He obtains the latter from the blood of the maternal portion of the umbilical cord, which is severed but not ligatured, and the blood
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 204 BACTERIOLOGY perature. The growth quickly extends, but ceases after the lapse of only a few days. Experiments have been carried on by Wertheim at the clinic of Professor P. Schauta in Vienna, on the cultivation of the gonococcus upon a medium consisting of a mixture of two or three parts peptone glycerine agar and one part human blood-serum. He obtains the latter from the blood of the maternal portion of the umbilical cord, which is severed but not ligatured, and the blood caught in sterilised flasks. In this way Wertheim was enabled to grow gonococci in plate-cultures, on which he was able to observe colonies visible to the naked eye even after twenty-four hours in the incubator. The microbes grow rapidly, displaying their characteristic forms and colour, and give the best evidence of their specific action by transmission to human beings. Wert- FlG. 79. — DEEP-LflNCi Colony of gonocoo- hcim s whole process vields astonishms ens ON A Sebtjji Agar a. ./ o Mmt ^'*"'""^ ^'^'^'" results, and according to his researches growth takes place decidedly quicker when deprived of oxygen than when the gas is admitted. The plate appears diffusely clouded in tAventy-four hours, and assumes the appearance of a delicate floeculent layer of moss. The majority of the colonies develop in the substance of the medium, the deep ones appearing whitish- grey by direct, yellowish-brown by transmitted light, and in three days show a peculiar nodulation, which is so regular as to recall the appearance of a blackberry (fig. 79). The superficial colonies have a compact dot situated exactly in the centre, which, when examined with a low power, is found to correspond in structure with the deeper colonies, but is surrounded on all sides by a superficial film which, though at first very delicate, transparent, finely granulated, and colourless, develops in th
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