. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FRESHWATER MUSSEL Na* AND Cl TRANSPORT 163 Results Chloride transport kinetics The rate of unidirectional Cr influx was dependent on the external C\ concentration for both pond water- acclimated (PWA) and salt-depleted (SD) C. fluminea (Fig. 1). When exposed to a range of NaCl concentrations, both PWA and SD animals displayed saturation kinetics. The cr uptake rate in PWA C. fluminea can be described by a Michaelis-Menten rectangular hyperbolic function [R > ) with the maximum C\ influx being the transport capacity (J
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FRESHWATER MUSSEL Na* AND Cl TRANSPORT 163 Results Chloride transport kinetics The rate of unidirectional Cr influx was dependent on the external C\ concentration for both pond water- acclimated (PWA) and salt-depleted (SD) C. fluminea (Fig. 1). When exposed to a range of NaCl concentrations, both PWA and SD animals displayed saturation kinetics. The cr uptake rate in PWA C. fluminea can be described by a Michaelis-Menten rectangular hyperbolic function [R > ) with the maximum C\ influx being the transport capacity (Jmax) and the Cl" concentration at half of the Jmax representing the transport affinity (Kn,). The J,„ax for PWA animals was ± //eq g"' dry tissue h~' and the K„, was ± niM. The CP influx in animals subjected to 2 weeks of salt depletion overlapped with that of PWA animals, and there was no significant difference between the two groups (P > ). The hemolymph Cl" and Na* concentrations in animals subjected to 2 weeks of salt depletion were ± mM and ± mM. respectively. There was no statistical difference in the Cl" and Na* concentrations from PWA C. fluminea stored in the lab for the same period of time ( ± mM and ± mM for Cl" and Na*. respectively, n = 25-30, f > ). Therefore, both data sets were combined to represent the Cl uptake kinetics for PWA animals. This combined transport capacity and the corresponding transport affinity were calculated to be ± ^eq g"' dry tissue h"' and ± mM. respectively. Salt depletion of 4 weeks or longer resulted in the elevation of unidirectional Cl uptake and a significant (P < ) reduction in hemolymph Cl" and Na* concen- trations ( ± mM and ± mM for Cl" and Na* after 4 weeks of SD. respectively, n = 25-30). The Cl" influx of the animals subjected to salt depletion for
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