. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. P T M-2 P T M-2. 21-5 14-4 Coomassie NBT Coomassie NBT M-2 |ig Protein 1-0 M-2 0-1 001 0 001 NBT Anti-Mefp-2 Figure 1. (Continued) acid glycoprotein (Sigma Chemical Co., St. Louis, Mis- souri) and collagen (type II calfskin) serving as positive controls. Denaturing isoelectric focussing (IEF) was performed with pH 3-10 ampholytes (FMC Bioproducts, Rockland, Maryland) in 8 M urea, 10% glycerol, 6% triton X-100, using M acetic acid and 1 N NaOH as the anolyte and catholyte, respectively (Guilian ct 1984). Gels were p
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. P T M-2 P T M-2. 21-5 14-4 Coomassie NBT Coomassie NBT M-2 |ig Protein 1-0 M-2 0-1 001 0 001 NBT Anti-Mefp-2 Figure 1. (Continued) acid glycoprotein (Sigma Chemical Co., St. Louis, Mis- souri) and collagen (type II calfskin) serving as positive controls. Denaturing isoelectric focussing (IEF) was performed with pH 3-10 ampholytes (FMC Bioproducts, Rockland, Maryland) in 8 M urea, 10% glycerol, 6% triton X-100, using M acetic acid and 1 N NaOH as the anolyte and catholyte, respectively (Guilian ct 1984). Gels were pre-equilibrated (200 V, 15 min; 500 V, 30 min; 800 V, 30 min) before samples (in the above buffer with 15 mg/ ml DTT) were applied to the gel under the anolyte. Pro- teins were focussed for 2-3 h at 1000 V, and a lane was removed for pH gradient determination (by incubation of cm gel slices overnight in 1 ml of 0.' °7- the gel was stained. 70 NaCl) before Results and Discussion Localisation of Mefp-2 in M. edulis byssus DOPA proteins extracted from serially sectioned mussel feet and electrophoretically resolved by AU-PAGE were detected by the NBT redox cycling assay and with Coo- massie Blue (Fig. la). Mefp-1 migrated as a doublet iden- tical to the previously characterized 130 kD DOPA protein (Waite. 1983), and it was found in foot sections through- out the length of the ventral groove corresponding to ac- cessory and phenol glands. Mefp-2 was exclusively asso- ciated with the distal foot section that encompasses the phenol gland involved in plaque secretion. Several minor NBT-positive proteins of intermediate electrophoretic. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952;
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
