. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. ISOLATION AND PURE-CULTURE METHODS 39 Sealing cultures. In order to seal the tubes permanently, sealing wax may be used after pushing the plug in somewhat below the level of the glass. Ordinary beeswax is also effective if a little ster- ile paraffin is first poured over the plug and permitted to harden. The length of life of a culture may sometimes be preserved in this way for several years. If the cultures are placed in a damp place, as in a closed box or case, with a surface of
. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. ISOLATION AND PURE-CULTURE METHODS 39 Sealing cultures. In order to seal the tubes permanently, sealing wax may be used after pushing the plug in somewhat below the level of the glass. Ordinary beeswax is also effective if a little ster- ile paraffin is first poured over the plug and permitted to harden. The length of life of a culture may sometimes be preserved in this way for several years. If the cultures are placed in a damp place, as in a closed box or case, with a surface of water evaporating, so as to diminish the loss of water from the tubes them- selves, it would be well to wipe out the case carefully with a dis- infectant before use. Where it is desired wholly to prevent evapora- tion under normal conditions of aeration a different method is nec- essary. The cultures may be put into a clean beaker or tin vessel fitted with a zinc screen, or cross wired with copper, serving to sep- arate the tubes from contact one with another. After thoroughly flaming the corks the vessel of tubes may be placed in a small dish or plate of water containing a little potassium dichromate and the whole covered with a clean bell glass. Cultures by sporophore frag- ments. In his studies upon Agar- iais campestris the writer ascer- tained that fragments of the inner tissue of the hymenophore of this fungus placed upon a sterile nutrient medium, such as bean pods, sterile compost, soil, etc., would readily develop a vigorous mycelium. In order to secure cultures of this particular species promptly, it was necessary (i) to use proper sterilization and. Fig. io. Culture op Polyporus sulphurews (Bull.) Fr., a Species Tough in Texture. (By Tissue Fragment Method). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the origina
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Keywords: ., bookauthorduggarbe, bookcentury1900, bookdecade1900, bookyear1909
