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. Ciba Foundation conferences on istopes in biochemistry;. Radioactive tracers in biochemistry; Isotopes -- Physiological effect. Protein and Polynucleotide Turnover 209 The maximum turnover in glycine in the proteins appeared approximately 30 hours later (60 hours after partial hepatec- tomy). All of the other amino acids also had a maximum at this point, indicating a common maximum of transamination. The cytological changes during regeneration (Fig. 6) are in accordance with earlier similar findings, and bear out the conclusions from tracer analyses that nucleic acid turnover and synthesis p
. Ciba Foundation conferences on istopes in biochemistry;. Radioactive tracers in biochemistry; Isotopes -- Physiological effect. Protein and Polynucleotide Turnover 209 The maximum turnover in glycine in the proteins appeared approximately 30 hours later (60 hours after partial hepatec- tomy). All of the other amino acids also had a maximum at this point, indicating a common maximum of transamination. The cytological changes during regeneration (Fig. 6) are in accordance with earlier similar findings, and bear out the conclusions from tracer analyses that nucleic acid turnover and synthesis play the primary role in nucleic acid-protein Nudeus-CytopjQsmic Ratio. 168 Hours after the operation. Nucleic acid Protein maxima. maximum. Fig. 6. Constituents of regenerating liver cell. In order to facilitate comparison, the curves have been separated by multi- plying each value by an arbitrary factor and adding a constant. The maximum values, expressed as percentage increase of normal liver cell constituents, are: nucleolus, 260; nucleus, 160; cyto- plasm, 145; and nucleus-cytoplasm ratio, 130. The mitosis frequency increased from less than per cent in normal liver to 10ii:0-32 per cent at 24 hours regeneration. formation. One notes the coincidence of the maximum rate of polynucleotide synthesis with the maximum of mitosis frequency and with the size of nuclei and nucleoli. Also it can be seen that during the period of maximum protein syn- thesis, at regeneration times 48-56 hours, these cytological quantities have already diminished. In this connection I would like to mention some measure- ments that have been made recently. The rats were fed by ISOTOPES 15. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation. Philadelphia, Blakiston
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