. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ()\|])\SI \( IIVI1 V IN SIIRIMI' I (i(iS 17. Figures 7-14. Trachypenaeus s/m///v eggs. HE, hatching envelope; PVS. perhitelline space; ATA, 3- Figure 7. Eggs in artificial seawater show a jelly layer (JL) outside of the retractile HE (HE) that surrounds the PVS (*). Bar equals 100 ^m. Figure 8. Scanning electron microscopy shows the HE covering the developing zygote to act as an environmental barrier. The HE collapses during processing, resulting in some wrinkling of the envelope. Bar equals 100 ^m. Fig
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ()\|])\SI \( IIVI1 V IN SIIRIMI' I (i(iS 17. Figures 7-14. Trachypenaeus s/m///v eggs. HE, hatching envelope; PVS. perhitelline space; ATA, 3- Figure 7. Eggs in artificial seawater show a jelly layer (JL) outside of the retractile HE (HE) that surrounds the PVS (*). Bar equals 100 ^m. Figure 8. Scanning electron microscopy shows the HE covering the developing zygote to act as an environmental barrier. The HE collapses during processing, resulting in some wrinkling of the envelope. Bar equals 100 ^m. Figure 9. Higher magnification of the HE shows an exterior series of ridges (arrowhead) not seen in Sicyonia ingenlis egg HEs. The ridges form octagonal patterns on the surface of the HE. Bar equals 1 nm. Figure 10. Transmission electron microscopy shows the distinct bilayered HE with the ridges ap- pearing to be constructed of the electron-dense material (DL). The arrowhead indicates a ridge now seen in cross section. Note the ring material in the PVS associated with the flocculent inner layer (large arrow). The flocculent layer is three to four times the thickness of the outer dense layer. Bar equals Mm. Figure 11. Phase microscopy of an egg in ATA shows a less refractile HE (HE) surrounding the PVS (*). Bar equals 100 nm. Figure 12. Scanning electron microscopy of an egg in ATA seawater shows an egg with more folds than in ASW. The collapse of the HE causes the envelope to be folded on itself. Bar equals 100 urn. Figure 13. At higher magnification, the ridges (arrowhead) are not as prominent as in the control eggs. The height of the ridges appears lower than in normal eggs. Bar equals 1 ^m. Figure 14. Transmission electron microscopy shows that the envelope is folded so that ridges (arrowheads) appear to be on the interior and exterior of the HE. The electron-dense layer is present, but the electron- translucent layer is greatly reduced. Bar equals Please note that
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
