. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EFFECT OF CO, ON FISH BLOOD 209 The stability of the more acid blood samples was checked by redetermining their oxygen capacity following treatment with carbon dioxide or lactic acid. For lactic acid samples, it was necessary to neutralize the acid by an equivalent amount of NaHCO3. Our experience has shown that some methemoglobin was likely to be formed in blood more acid than approximately pH , and that it was necessary to make allowance for this in calculating the percentage HbOo present in these samples. Methemoglobin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EFFECT OF CO, ON FISH BLOOD 209 The stability of the more acid blood samples was checked by redetermining their oxygen capacity following treatment with carbon dioxide or lactic acid. For lactic acid samples, it was necessary to neutralize the acid by an equivalent amount of NaHCO3. Our experience has shown that some methemoglobin was likely to be formed in blood more acid than approximately pH , and that it was necessary to make allowance for this in calculating the percentage HbOo present in these samples. Methemoglobin formation proved to be more trouble- some with hemolvzed blood than with the whole blood. RESULTS It can be seen from Figure 1 that whole blood begins to lose oxygen-combining power rapidly as the pH falls below (carbon dioxide tension about 2 mm. Hg). CD I. WHOLE BLOOD I 1 1 1 400 200 100 50 1 10 20 PCQHEMOLrZED BLOOD 150 100 50 10 4 2 Prn WHOLE BLOOO C02 i i i i 1 1 62 64 7 2 7,8 PH FIGURE 1. The relation between oxygen-combining power of hemoglobin at 155 mm. O2- pressure and pH in whole and hemolyzed blood of the tautog at 15° Centigrade. The pH was modified by the addition of carbon dioxide, the approximate tensions being indicated in the graph, and was calculated by means of the Henderson-Hasselbalch equation. Hemolyzed blood, on the contrary, shows little loss in oxygen-combining power until the pH goes below (carbon dioxide tension approximately 100 mm. Hg). This is in agreement with Hall and McCutcheon's (1938) observation that f\J O 60 CD I 40. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore,
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