. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MIDP1ECES OF SEA URCHIN SPERM 299 drion and the plasma membrane (Afzelius and Mohri. 1966). Although similar lipid globules have not been ob- served in spermatozoa of other sea urchin species, it has been reported that lipid bodies are present in A. punctulata (Longo and Anderson, 1969) and A. lixitla (Cosson and Gulik, 1982) spermatozoa. The lipid body differs from the lipid globules, because the former is located inside the mitochondrion and it is relatively smaller than lipid glob- ules (Longo and Anderson, 1969; Cosson a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MIDP1ECES OF SEA URCHIN SPERM 299 drion and the plasma membrane (Afzelius and Mohri. 1966). Although similar lipid globules have not been ob- served in spermatozoa of other sea urchin species, it has been reported that lipid bodies are present in A. punctulata (Longo and Anderson, 1969) and A. lixitla (Cosson and Gulik, 1982) spermatozoa. The lipid body differs from the lipid globules, because the former is located inside the mitochondrion and it is relatively smaller than lipid glob- ules (Longo and Anderson, 1969; Cosson and Gulik, 1982). In the present study, the midpieces of H. pulcher- rimus spermatozoa were examined ultrastructurally to clarify further the energy metabolism of sea urchin sper- matozoa. prefixed in glutaraldehyde ASW solution for 40-60 min at 4°C; a volume of sperm suspension was mixed with the same volume of cold 5% glutaraldehyde in 80% ASW. The prefixed spermatozoa were rinsed with cold ASW and post-fixed with 1% OsO4 in ASW for 2 h at 4°C. Samples were washed in distilled water, and then immersed in saturated aqueous uranyl acetate for 1 h for block staining. After dehydration in a graded series of ethanol solutions, the specimens were embedded in epoxy resin and ultrathin sections were cut on a Reichert Ultra- cut ultramicrotome. After staining the specimens with lead citrate, we used a Hitachi 7000 or JEM 100 CX electron microscope to observe them. Materials and Methods Materials Spawning of stored spermatozoa of the sea urchin H. pii/c/ierrimus was induced by injecting M KC1 into the coelomic cavity. Semen was always collected freshly as "dry sperm" and kept undiluted on ice. Incubation of spermatozoa Dry sperm were diluted 100-fold in artificial seawater (ASW) consisting of 458 mA/NaCl, mM KC1, 10 mM CaCl:, 49 mM MgSO4, and 10 mM Tris-HCl, pH After dilution and incubation at 20°C, the sperm suspen- sion was centrifuged at 3000 X g for 5
Size: 1423px × 1755px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
