. Control mechanisms in cellular processes. Cytology; Biochemistry. 10 CONTROL MECHANISMS IN CELLULAR PROCESSES ponent that behaves similarly to a component in highly pm-ified preparations of wild-type Tsase (Carsiotis et ah, 1960). This com- ponent can be resolved on starch electrophoresis and by the use of antigen-antibody reactions in agar gel. The latter is shown in Fig. 1-2. Perhaps this component in the CRM-less mutant bears some structural relationship to Tsase. Clearly, many types of mutationally altered proteins are formed by Tsase mutants, and it becomes of considerable importance to
. Control mechanisms in cellular processes. Cytology; Biochemistry. 10 CONTROL MECHANISMS IN CELLULAR PROCESSES ponent that behaves similarly to a component in highly pm-ified preparations of wild-type Tsase (Carsiotis et ah, 1960). This com- ponent can be resolved on starch electrophoresis and by the use of antigen-antibody reactions in agar gel. The latter is shown in Fig. 1-2. Perhaps this component in the CRM-less mutant bears some structural relationship to Tsase. Clearly, many types of mutationally altered proteins are formed by Tsase mutants, and it becomes of considerable importance to. Fig. 1-2. Antigen-antibody reactions in agar gel. Reservoirs: c. anti-Tsase, partially absorbed with fractions of mutant tdj; 1. unabsorbed anti-Tsase; 2. Tsase having a specific activity of 225; 3. Tsase having a specific activity of 1940; 4. unabsorbed anti-Tsase. establish whether mutants with similar protein damage possess de- fects in the same genetic region. On the basis of interallelic crosses in N. crassa and transduction mapping in E. coli, the fine structure of the gene controlling Tsase formation in both organisms is being analyzed. In agreement with the concept of mutations primarily causing substitutions in amino acid sequence, and subsequently in the conformation of the protein, it was found that CRM mutants which produce altered A-proteins with similar properties were clus- tered in particular genetic areas and were not distributed at random throughout the entire Tsase genetic region (Yanofsky and Crawford, 1959; Yanofsky, 1960). From the work of Suyama and Bonner at Yale, it appears that Neurospora CRM mutants which retain the InGP ;=i In + TF reaction are localized in one region of the genetic. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Bonner, David M; Society of General Physiologist
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