. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 139 studied. So far as we could determine, the nerve cells in the 4 months starved ganglia were normal and presented all of the usual nerve cell features. The appearance of the cells in the 16 months starved was con- fusing for some time. The nucleus seemed normal and was surrounded by a fine granular cytoplasm in which was suspended a golden-yellow oil droplet which gave the fat reaction with Sudan III. We finally were able to dissect out a nearly complete living nerve cell which is shown in photo 5. Here the cell wall is cl
. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 139 studied. So far as we could determine, the nerve cells in the 4 months starved ganglia were normal and presented all of the usual nerve cell features. The appearance of the cells in the 16 months starved was con- fusing for some time. The nucleus seemed normal and was surrounded by a fine granular cytoplasm in which was suspended a golden-yellow oil droplet which gave the fat reaction with Sudan III. We finally were able to dissect out a nearly complete living nerve cell which is shown in photo 5. Here the cell wall is clearly indicated as a sharp line, the nucleus, cytoplasm and oil-droplet are in contact with the cell wall for part of the way but toward the main mass of cells there is indicated a space which is free from granules, etc. This we inter- pret as the effect of the pro- longed starvation, the cavity free from the granules being occupied probably by lymph. Cells fixed and subsequently stained show exactly cor- responding conditions. Stomach. In making the histological preparations care was taken to have the conditions uniform through- out. The material here described was all fixed in Bouin's fluid, hardened in alcohol, sectioned in paraffin and stained in iron haemato- xylin and eosin. The sections were uniformly cut at 4 micra. The pieces sectioned were taken from corresponding parts of the various organs. When the sections are examined the first striking observation made is that in the specimen starved for the longer period there has been more of shrinkage than in the more fortunate and less starved individual. The whole stomach wall is somewhat reduced in thickness, the muscle layers, sub-mucosa and mucosa all being affected. Photo- graphs 6 and 7 indicate the conditions shown in the outer portion of. Fig. 5. Photograph of an unstained, living nerve cell dissected from the dorsal ganglion of the Necturus starved for 16 months, cw cell wall, Nil nucleus, F fat drop. Vac vacuole. N
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