. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 430 MARIA DOLORES PEREZ-GONZALEZ effect on the chromatophores of legs of Uca No significant loss of activity was observed in solution B after the high speed centrifugation. However, solution C, the suspension of a presumably invisible sediment, caused a small effect on the chromatophores (Fig. 2). This fact is indicative of some sedimentation of gran- ules and from these results it is not possible to infer how much of the hormone is present in granules and how much is found free in the homogenate. It is prob- able t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 430 MARIA DOLORES PEREZ-GONZALEZ effect on the chromatophores of legs of Uca No significant loss of activity was observed in solution B after the high speed centrifugation. However, solution C, the suspension of a presumably invisible sediment, caused a small effect on the chromatophores (Fig. 2). This fact is indicative of some sedimentation of gran- ules and from these results it is not possible to infer how much of the hormone is present in granules and how much is found free in the homogenate. It is prob- able that for a complete sedimentation a longer and higher-speed centrifugation is necessary. An indication of the percentage of hormone contained in granules in isotonic sucrose is given by the analysis of the activity of homogenates in isotonic sucrose and distilled water after a series of dilutions. Figure 3 shows the results of injec- tions of ml. of homogenates of 2 sinus glands in 1 ml. of M sucrose and in 1 ml. of distilled water diluted 10, 100 and 1000 times in M" sucrose and sea (T §3 CL O I2 <r x o I. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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