. dan bai zhi zu xue cong xu lie dao gong neng. botany. 32 èç½è´¨ç»å-¦ æ»ä¹ï¼SAGE被广æ³åºç¨äºä»»ä½çç©å-¦ä½ç³»ãç»åèªå¨DNAåºååæå足å¤çç ç©ä¿¡æ¯å-¦æ¯æï¼å®æ¯ä¸ç§ç¨äºmRNA丰度å®éçææè精确çæ¹æ³ å·®å¼æ¾ç¤ºPCR (DD-PCR) å·®å¼æ¾ç¤ºPCRæ¯ä¸ç§ç¡®å®ä¸¤ä¸ªçç©å-¦æ ·åå·®å¼è¡¨è¾¾çcDNAç段çæ¹æ³ï¼Liang and Pardee, 1992)(è§å¾)ãè¿ä¸æ¹æ³çåºç¡æ¯ç¨ä¸¤ä¸ªå¯¡æ ¸æé ¸å¼ç©ä»mRNA产ç cDNAç段ï¼ä¸ä¸ªæ¯ä¸è½¬å½ç©çpolyAå°¾äºè¡¥[å¦oHgo-d(T)iiVN]ï¼å¦ä¸ä¸ªæ¯ç-çéæº æ ¸é ¸åºåï¼ä¾å¦10å¯¡æ ¸è·é ¸ï¼ãå½æ足å¤çå¼ç©ç»åæ¶ï¼DD-PCRè½ç¡®å®ä¸ä¸ªçç©å-¦æ · åä¸-ææç转å½ç©ãcDNAåæåï¼ç段å¨PCRæ©å¢è¿ç¨ä¸-被æ è®°ï¼æ¾å°æ§åä½ç´ æ è§å æ è®°ï
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