. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 W. H. McSHAN, SOL KRAMER AND N. F. OLSON As has been found for the ATPase of other kinds of tissue Mg and Mn ions were also found to activate the ATPase of muscle from the male and female cockroach. The degree of activation with different concentrations of Ca, Mg and Mn ions is shown in Figure 4. The ATPase activity was more nearly the same when the concentration of each of the ions was X 10~3 M. Mg ions were more effective in activating the system than Ca and Mn ions at a concentration of X 1O3 M. Similarly, Sack
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 50 W. H. McSHAN, SOL KRAMER AND N. F. OLSON As has been found for the ATPase of other kinds of tissue Mg and Mn ions were also found to activate the ATPase of muscle from the male and female cockroach. The degree of activation with different concentrations of Ca, Mg and Mn ions is shown in Figure 4. The ATPase activity was more nearly the same when the concentration of each of the ions was X 10~3 M. Mg ions were more effective in activating the system than Ca and Mn ions at a concentration of X 1O3 M. Similarly, Sacktor (1953) found that Mg ions were more effective than Ca ions at a concentration of 1 X 10~3 M. Ca ions were more effective in activating the ATPase of cockroach muscle at concentrations greater than X 10'3 M. oca Mn+. M IONS (xlCT FIGURE 4. Activation of the ATPase of cockroach muscle by increasing concentrations of Ca, Mn and Mg ions. On the basis of the above results ml. of M CaCl,-2H2O and ml. of M ATP ( X 10'3 M final concentration of each) were used in the system for the determination of the ATPase of cockroach and woodroach muscle. The amounts of the other reagents used in the system were: ml. of M sodium diethylbarbiturate buffer of pH and, as indicated above, , and ml. of one per cent homogenate plus sufficient water to give a total volume of ml. for incubation. Three amounts of tissue were used for each determination to demonstrate that the phosphate released from the ATP was linearly related to the ATPase of these amounts of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
