. The Biological bulletin. Biology; Zoology; Marine biology. 266 S. BINGHAM AND J. A. SCHIFF M0O4 REMOVED: 0 HRS. 22 26 32 TIME (HOURS) Figure 7. Reversal of molybdate inhibition of attachment when molybdate was removed and sulfate added after various times of incubation with molybdate. Cells were released by grinding at 4° C in a medium lacking sulfate and calcium but containing M molybdate and were held at 4° C in darkness for the time indicated. At 0, 2, 8, and 24 hr of incubation with molybdate, an aliquot was removed, centrifuged at 121 g for 3 min at 4° C, the cells were resuspend
. The Biological bulletin. Biology; Zoology; Marine biology. 266 S. BINGHAM AND J. A. SCHIFF M0O4 REMOVED: 0 HRS. 22 26 32 TIME (HOURS) Figure 7. Reversal of molybdate inhibition of attachment when molybdate was removed and sulfate added after various times of incubation with molybdate. Cells were released by grinding at 4° C in a medium lacking sulfate and calcium but containing M molybdate and were held at 4° C in darkness for the time indicated. At 0, 2, 8, and 24 hr of incubation with molybdate, an aliquot was removed, centrifuged at 121 g for 3 min at 4° C, the cells were resuspended in an equal volume of Lewin's medium and attachment was assayed at 23° as a function of time. These experiments show that the molybdate inhibition of attachment resembles the known effects of molybdate on sulfate activation (Wilson and Bandurski, 1958; Ramus, 1974). The inhibition of both processes is competitive and is 24 36 90 J tâ ⢠2~ 2~ â M0O4, + SO4 P 70 J50 X 0 jl 30 < 10 s^â """"âA -Aâ_ 0 p p ° â M0O4, âSO4 â âA . 7- âA2- + M0O4 (AT 24 HRS), + SO4 " A __^ 2" * 2" + M0O4 (AT 24HRS),-S04 96 TIME (HOURS) Figure 8. Reversal of attachment of single cells on addition of molybdate to already attached cells. All cells were allowed to attach in the normal manner. At 24 hr the medium in each of several dishes was removed and replaced with Lewin's medium (minus calcium) with additions or deletions as shown. At various times thereafter, coverslips were rinsed in the usual manner and fields counted to estimate the loss of attached Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological
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