. Pathological technique; a practical manual for workers in pathological histology and bacteriology. t. 4. Fatty Detritus.—Fat-drops are frequently found, due tothe fatty degeneration of cells. 5. Elastic fibers (Fig. 151) occur singly, but more often asa network. They are recognized by their sharp, dark out- 30 466 PA THOL 0 GICA L TE CHNIQ UE. lines due to their high degree of refractiveness, and by theirmarked degree of resistance to acids and alkalies by whichother like tissues, such as connective-tissue fibers, are de-stroyed. Elastic fibers are most abundant in the caseousmasses above me
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. t. 4. Fatty Detritus.—Fat-drops are frequently found, due tothe fatty degeneration of cells. 5. Elastic fibers (Fig. 151) occur singly, but more often asa network. They are recognized by their sharp, dark out- 30 466 PA THOL 0 GICA L TE CHNIQ UE. lines due to their high degree of refractiveness, and by theirmarked degree of resistance to acids and alkalies by whichother like tissues, such as connective-tissue fibers, are de-stroyed. Elastic fibers are most abundant in the caseousmasses above mentioned. When these masses cannot befound, the thicker portions of the sputum are squeezed be-tween a slide and cover-glass or between two slides, and ex-amined with a low power. The examination is renderedeasier by mixing a little sputum with a 10 per cent, solutionof caustic potash or soda. In certain cases it is necessaryto mix together equal parts of the sputum and 10 per cent,caustic potash or soda, and to boil the mixture until thesputum is dissolved. The solution is then mixed with four. Fig. 151.—Elastic fibers (after Striimpell). times its own volume of water and allowed to stand fortwenty-four hours, when the sediment can be examined forthe elastic fibers. Vegetable and Animal Parasites.—Of the vegetableparasites, the most important is the tubercle bacillus (for itsexamination see page 348). Other bacteria sometimes ex-amined for are the pneumococcus, the influenza bacillus, andactinomyces. W. H. Smiths Method of Staining Bacteria in Sputum.—This has been found particularly useful in demonstrating thepneumococcus in the sputum, The sputum or other materialshould be fresh. The cover-glasses should be spread asthinly as possible and fixed by passing three times throughthe flame in the usual manner. I. Stain in aniline-gentian-violet solution for a fewseconds, gently warming until the staining fluid steams. CLINICAL PATHOLOGY. 467 2. Wash in water. 3. Cover with
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