. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ION REGULATION IN TETRAHYMENA 131 amounts of the appropriate radioisotope, K42 or Na24, obtained from Oak Ridge National Laboratories as chlorides in HC1 solution, and neutralized with NaOH before use. Counts per minute of wet samples were determined with an end- window counter or a Nal-Tl crystal scintillation well counter. Per cent exchanges of intracellular K and Na were calculated from the specific activities of the cells and of the medium. 40 30 £» CT O> E oC ~ 10. OD Ko'.mM t 5 10 20 30 FIGURE 1. K and Na content of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ION REGULATION IN TETRAHYMENA 131 amounts of the appropriate radioisotope, K42 or Na24, obtained from Oak Ridge National Laboratories as chlorides in HC1 solution, and neutralized with NaOH before use. Counts per minute of wet samples were determined with an end- window counter or a Nal-Tl crystal scintillation well counter. Per cent exchanges of intracellular K and Na were calculated from the specific activities of the cells and of the medium. 40 30 £» CT O> E oC ~ 10. OD Ko'.mM t 5 10 20 30 FIGURE 1. K and Na content of Tctrahymena in normal and diluted media. Ordinate: cellular concentrations of K and Na ( cells); abscissas: concentrations of K and Na in the medium (MIA/). Open circles: Ki; solid circles: Nai. Points for Ki and Nai in normal medium (see arrows) are averages of 44 determinations; vertical lines delimit the total range of the determinations in normal medium. RESULTS Table II shows the K and Na content of cells in normal medium, and the K, Na and Cl content of normal medium. The volume and weight of an average cell in normal medium were X 1(H ^1. and X 1O2 /*g., respectively, as determined from cell counts and packed cell volumes (corrected for Risa space). Dry weight of cells in normal medium was determined to be of wet weight, so cells are water. The percentage of Risa space in a volume of packed cells in normal medium was 15% (eight determinations ranging from 14% to 18%, SE = ). This value was not significantly different for cells equilibrated with medium diluted Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattr
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