. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 446 H. BURR STEINBACH chloride. A little potassium is lost on soaking and calcium increases slightly. The main purpose of this work was to follow the loss or gain of cellular electrolytes, using the chloride space (ratio of Cl inside to Cl outside) as a measure of the extracellular space of the whole tissue. In order to do this, it was first essential to show that chloride in the tissue bore a simple linear relationship to the chloride concentration of the medium. Muscles were soaked in various dilutions of sea water with is
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 446 H. BURR STEINBACH chloride. A little potassium is lost on soaking and calcium increases slightly. The main purpose of this work was to follow the loss or gain of cellular electrolytes, using the chloride space (ratio of Cl inside to Cl outside) as a measure of the extracellular space of the whole tissue. In order to do this, it was first essential to show that chloride in the tissue bore a simple linear relationship to the chloride concentration of the medium. Muscles were soaked in various dilutions of sea water with isotonic sucrose solutions and then analyzed. A few experiments 30- 25- 20 1C 15' 10 5- -t. E0%_ 10 15 20 25 30 Meq % in solution 35 40 50 55 FIG. 1. Chloride (•) and sodium (O) concentrations in Phascolosoma mus- cles plotted against the same elements in solution. The concentrations in the solutions were altered by diluting normal sea water with isotonic buffered sucrose solutions. Tissues soaked 2 to 4 hours before analysis. The cross indicates the minimal salt concentration for maintaining irritability (response to electrical stimulation). All concentrations in milliequivalents per hundred grams solution or final wet weight of tissue. Straight lines represent calculated values for chlo- ride space as indicated. Each point is a single determination on several muscles. were also done in which normal sea water was diluted with an artificial sea water (Allen's formula) made up with nitrates instead of chlorides. The results (Fig. 1) show clearly that chloride in the muscle does bear a simple linear relationship to chloride of the medium and hence the assumption of an extracellular chloride space is probably correct. On this basis, about 30 per cent of the whole tissue is extracellular space. Determinations of sodium were made on muscles similarly treated and since sodium appears to be extracellular in soaked muscles the results are included in Fig. Please note that these ima
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