. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 300 F. LYNEN, S. OCHOA VOL. 12 (1953) that the active intermediates might be the CoA derivatives of the fatty acids^'-^'^". This behef was reinforced by the observation that S-acetyl CoA or the S-acyl derivatives of higher fatty acids can be generated enzymically through a reaction ot the fatty acid with acyl phosphates in the presence of CoA^^-eo or with CoA and ATPii'44'i5,36,49,72,23,i6,4i,6-_ Finally, work on the enzymic breakdown and sy
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 300 F. LYNEN, S. OCHOA VOL. 12 (1953) that the active intermediates might be the CoA derivatives of the fatty acids^'-^'^". This behef was reinforced by the observation that S-acetyl CoA or the S-acyl derivatives of higher fatty acids can be generated enzymically through a reaction ot the fatty acid with acyl phosphates in the presence of CoA^^-eo or with CoA and ATPii'44'i5,36,49,72,23,i6,4i,6-_ Finally, work on the enzymic breakdown and synthesis of acetoacetate and other /3-keto fatty acids'^''^'''^'^^''^'^'"*'^^'^'2^'^^'2- opened the way for an understanding of the mechanism whereby fatty acid chains are shortened or elongated, by the removal or addition of acetyl CoA, during fatty acid oxidation or synthesis. By employing synthetic S-acyl analogues of the fatty acid derivatives of CoA"*^- ^^ or the CoA fatty acid derivatives themselves^-^^'^^ more recently made available by chemical or enzymatic synthesis, it has been possible to characterize and isolate from animal tissues some of the enzymes of fatty acid metabolism and obtain a clearer picture of the process as a whole. The development of rapid and sensitive optical methods of assay, whose introduction in enzymology we owe to Otto Warburg, has greatly facilitated the task of purifying the individual enzymes and studying their mechanism of action. The work described in this paper owes much to Warburg's pioneering contri- butions which opened up new avenues of approach to dynamic biochemistry. Fatty acid cycle The results of the work summarized above and of the more recent work to be discussed in this paper show that fatty acid oxidation and synthesis proceed through the reactions illustrated in Fig. i. Fatty acid synthesis is accomplished through repetition of a cycle of four con- secutive reactions: (a) condensa- tion of two molecules of acetyl C
Size: 1891px × 1321px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
