. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ^. ' B m c '^ \ p • • , g <i* '* f - * I ! - ., :•% m : &:$t. ' !• ^ • •'•-*• vwlfN H \ • '.#'':'•• :::-- • . <£ - " - '""" "" •'-- ¥**$&, .-'? ...-:: '» - •'- S-/-VH,; -- ^.JW^:' '.i^l Figure 5. Electron micrographs of immune and pre-immune treatments of ciliated duct epithelium. (A & B) Low magnification of the ciliated duct epithelium in: (A) longitudinal section, and (B) cross section, showing numerous cilia and ciliary root hairs (arrow) (fixed for TEM. not for immunocytoc
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ^. ' B m c '^ \ p • • , g <i* '* f - * I ! - ., :•% m : &:$t. ' !• ^ • •'•-*• vwlfN H \ • '.#'':'•• :::-- • . <£ - " - '""" "" •'-- ¥**$&, .-'? ...-:: '» - •'- S-/-VH,; -- ^.JW^:' '.i^l Figure 5. Electron micrographs of immune and pre-immune treatments of ciliated duct epithelium. (A & B) Low magnification of the ciliated duct epithelium in: (A) longitudinal section, and (B) cross section, showing numerous cilia and ciliary root hairs (arrow) (fixed for TEM. not for immunocytochemistry. as described in Methods). Bars = 1 ^m. (C) High magnification of immune-treated duct epithelium cytoplasm revealing numerous 15 nm gold spheres. Bar = ^m. ID) High magnification of pre-immune-treated duct epithelium cytoplasm revealing few 15 nm gold spheres. Bar = ^m. 2). Such a high abundance of L-crystallin together with an apparent reduction in the production of proteins typical of a muscle tissue, such as actin and myosin (Montgomery and McFall-Ngai, 1992) and a lack of subcellular structure (Fig. 3; McFall-Ngai and Montgomery, 1990) in this pre- dominant cell type suggests that these cells do little else than synthesize and retain L-crystallin. As with ocular lens tissue, the over-production of one or a few proteins in specialized lens cells is thought to result in a tissue with a high refractive index, which results in increased trans- parency (Bloemendal. 1981). L-crystallin abundance appears to vary along the me- dial/lateral axis in some sections (Fig. Ib). but not in oth- ers. The lighter staining lateral portion of the lens could indicate a differential abundance of L-crystallin along this axis in some areas of the lens, or could be due to uneven fixation of the tissue. L-crystallin occurrence is uniform through the lens along the dorsal/ventral axis, except for the dorsal lens margin where lens cells appear to give w
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
