The house-fly, Musca domestica Linn: its structure, habits, development, relation to disease and control . 97. Agar-agar slope culture of bacteria and moulds deposited by M. domenticacaught in the authors laboratoiy (.Jan. 1910) and allowed to make a singlejourney over the culture medium. hour agar slope of Bacillus typhosus recently obtained from anenteric stool and rubbed Tip with fine soil. This was introducedwith some infected honey into a cage of flies together with sterilelitmus agar plates and dishes containing sterile broth, which wereplaced at a short distance from the infected soil a
The house-fly, Musca domestica Linn: its structure, habits, development, relation to disease and control . 97. Agar-agar slope culture of bacteria and moulds deposited by M. domenticacaught in the authors laboratoiy (.Jan. 1910) and allowed to make a singlejourney over the culture medium. hour agar slope of Bacillus typhosus recently obtained from anenteric stool and rubbed Tip with fine soil. This was introducedwith some infected honey into a cage of flies together with sterilelitmus agar plates and dishes containing sterile broth, which wereplaced at a short distance from the infected soil and honey. Flieswere seen to settle on the infected matter and on the agar and 248 THE CARRIAGE OF TYPHOID FEVER BY FLIES broth. The agar plates and broth were removed after a few days,and after incubation at 37° C. for twenty-four hours colonies ofBacillus typhosus were found on the agar plates, and the bacilluswas recovered from the broth. In a further experiment the in-fected material was dusted over with fine earth to representsuperficially buried dejecta, and the bacillus was isolated from agar. Fig. 98. Agar plate culture of tracks ot .1/. ilumestica caught in a room and allowedto walk across and around the medium. Natural size. (Prepared by H. ) plates upon which the flies had subsequently walked, as in theformer experiment. They also found the bacillus on the heads,wings, legs and bodies of flies which had been allowed to haveaccess to infected material. Hamilton (1903) recovered Bacillus typhosus five times ineighteen experiments from flies caught in two imdrained privies. EXPERIMENTS WITH BACILLUS TYPHOSUS 249 on the fences of two yards, on the walls of two houses, and in theroom of an enteric fever patient. Ficker (1903) found that when flies were fed upon typh(jidcultures they could contaminate objects upon which they typhoid bacilli were present in the head and on the wings andlegs of the fly five days after feeding. He also recovered B. ty-jjI
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