. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTHORACIC GLANDS OF LEUCOPHAEA 189 less folded, with the result that the width of the organ varies. In the wider por- tions 8 to 12 nuclei may be counted across the width of the cellular layer of one side (Fig. 3). The deeply staining nuclei are the most prominent feature of the gland. They are ovoid and, for the most part, approximately uniform in size and appearance. In regard to their dense arrangement and general morphology they resemble the nuclei of the corpora allata of the same species. In some nymphal glands a num
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PROTHORACIC GLANDS OF LEUCOPHAEA 189 less folded, with the result that the width of the organ varies. In the wider por- tions 8 to 12 nuclei may be counted across the width of the cellular layer of one side (Fig. 3). The deeply staining nuclei are the most prominent feature of the gland. They are ovoid and, for the most part, approximately uniform in size and appearance. In regard to their dense arrangement and general morphology they resemble the nuclei of the corpora allata of the same species. In some nymphal glands a number of considerably larger nuclei may be observed. The majority of specimens studied showed some pycnotic nuclei in the prothoracic glands. f .'i -*-. *v»~ C O NNECT *.*v'iS$<*rf£:3f TISSUE " "1". , ... SHEATH %fs*' ^,"^"'° ^ rt"O»^ ,,-tv & »-~ ' -- *rtr ,-.•• V: s?*i. FIGURE 4. Prothoracic gland of last instar female nymph of Leucophaea in longitudinal section. Paraffin, 4 micra, Mallory azan. The cytoplasm is not abundant and the cell boundaries are difficult to discern, except under favorable conditions as, for instance, in the periphery of the sectioned organ, where the nuclei are less densely packed. With the methods employed for permanent preparations no appreciable amount of cytoplasmic inclusions indicating a secretory activity of the prothoracic gland cells was observed. In two specimens a few acidophilic granules were seen, in others the cytoplasm showed vacuolization. These observations suggest the possibility that certain cytoplasmic inclusions may be dissolved during the ordinary embedding and staining procedure. This view is further supported by the results of supravital staining. The cytoplasm of fresh tissue subjected to supravital dyes for a suitable period of time contains inclusions, as indicated in Figure 5. Round bodies varying in diameter stain a distinct blue with methylene blue. The staining is not always uni
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
