. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 JOSEPH G. KUNKEL 1-2 2. O) UJ z O 00 0-8 Q U Ld 0-4 UJ 00 0 ^«>, CONTROLS -^o^AUTOTOMISED autotomy i. 4 8 12 16 DAYS AFTER FEEDING FIGURE 5. Serum ecdysone levels in normal and autotomized sixth instar larvae of Blatta oricntalis. Autotomy of a metathoracic femur-tibia-tarsus was performed at the indi- cated time. A five microliter sample of hemolymph was taken from each of five animals randomly selected from mass synchronized cultures. Ecdysone was measured on individual blood samples by radiommune assay. The average
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 156 JOSEPH G. KUNKEL 1-2 2. O) UJ z O 00 0-8 Q U Ld 0-4 UJ 00 0 ^«>, CONTROLS -^o^AUTOTOMISED autotomy i. 4 8 12 16 DAYS AFTER FEEDING FIGURE 5. Serum ecdysone levels in normal and autotomized sixth instar larvae of Blatta oricntalis. Autotomy of a metathoracic femur-tibia-tarsus was performed at the indi- cated time. A five microliter sample of hemolymph was taken from each of five animals randomly selected from mass synchronized cultures. Ecdysone was measured on individual blood samples by radiommune assay. The average ecdysone titer for each day is plotted for each group. The average times of ecdysis, E, of the donor cultures are indicated by arrows. each animal has an all or none component; that is, either an animal delays its molting cycle and regenerates a limb or it neither de-lays molting nor regenerates (Kunkel, 1975b). In addition, the animals which are prevented by the exogenous ecdysone from delaying their molting cycle to regenerate exhibit graded dose dependent responses to the injected ecdysone (Fig. 7). Below 1 fig/animal, no animals showed any response to ecdysone. At the lower doses of 1 and 2 pg/ animal, the larvae which did not delay their molting cycles underwent ecdysis beginning at the same time as the unautotomized controls. At the higher doses, 4 and 8 /xg/animal, the larvae undergo ecdysis at successively earlier times, such that at the 8 /xg/animal close the median time of ecdysis is days earlier than the unautotomized controls. This ability to accelerate the timing of ecdysis to >'iit degrees is circumstantial evidence that the action of ecdysone is spread a substantial time period in the individual cockroach. The low doses of :>ne may have an effect comparable to the suggested early peak or rise of ecdysone which follows the brain critical period. The higher doses of ecdysone may be having an effect comparable to the major peak of endogenous ecdysone, by
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
