. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHARACTERIZATION OF CORAL MORPHOGEN 111 100 - _ 80 - + Agarase tn O 9- o E 3 QJ 60 -. 0246 + Agarase, 1 sample) No Enzyme! No Inducer J "j 10 12 14 16 18 20 22 Digestion (hr) Figure 7. Enzymatic solubilization and further purification of small morphogen. The insoluble, cell wall-associated inducer of larval meta- morphosis was digested inside a dialysis tubing (retention limit = 14,000 Da) with purified agarase. Small morphogens released from the insoluble inducer were allowed to accumulate in the external dialysate, wh
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHARACTERIZATION OF CORAL MORPHOGEN 111 100 - _ 80 - + Agarase tn O 9- o E 3 QJ 60 -. 0246 + Agarase, 1 sample) No Enzyme! No Inducer J "j 10 12 14 16 18 20 22 Digestion (hr) Figure 7. Enzymatic solubilization and further purification of small morphogen. The insoluble, cell wall-associated inducer of larval meta- morphosis was digested inside a dialysis tubing (retention limit = 14,000 Da) with purified agarase. Small morphogens released from the insoluble inducer were allowed to accumulate in the external dialysate, which was removed and assayed, and replaced with fresh external medium, every two hours. An otherwise identical sample was digested in parallel with no change of dialysate for 22 h, after which the external medium was removed and assayed (dotted line). Other details as described in the text; all assays were performed in duplicate under standard conditions. Con- trols conducted in parallel included assays of dialysates changed every 2 h from a sample incubated with no enzyme, and larvae incubated with no additions; these gave 0 ± 0% metamorphosis. Results show meta- morphosis induced by the dialysates. 6). The external dialysates (including the permeant small morphogens released) were allowed to diffuse and accu- mulate outside of the membranes for a fixed interval, after which they were removed for assay, and replaced with fresh external liquid for the next interval of incubation. With purified agarase, and 2-h intervals for accumulation, removal, and replacement of the external dialysates. we observed a time-dependent increase and subsequent de- cline in the rate of appearance of dialyzable morphogenic activity with M < 14,000 Da (Fig. 7). However, far less of the total morphogenic activity could be detected in an otherwise identical incubation, in which only one sample of dialysate was collected and assayed after prolonged in- cubation (24 h). All of the dialysates were hel
Size: 1438px × 1738px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
