. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 5. Scanning electron micrographs of light organs (right half only) of 4-day old squid exposed upon hatching to: (A) CSW for 4 days; (B) ESI 14 for 12 h followed by treatment with Cm for days; (C) ESI 14-U2 (Cm resistant strain) for 12 h followed by treatment with Cm for days. See Figure 3A for experimental design. Scale bar = 100 urn. Squid exposed to I '.fischeri for 12 h showed regression of the CEA similar to that of squid exposed for 4 days (Fig. 5b). Negative control animals exposed for 4 days to CSW (Fig
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 5. Scanning electron micrographs of light organs (right half only) of 4-day old squid exposed upon hatching to: (A) CSW for 4 days; (B) ESI 14 for 12 h followed by treatment with Cm for days; (C) ESI 14-U2 (Cm resistant strain) for 12 h followed by treatment with Cm for days. See Figure 3A for experimental design. Scale bar = 100 urn. Squid exposed to I '.fischeri for 12 h showed regression of the CEA similar to that of squid exposed for 4 days (Fig. 5b). Negative control animals exposed for 4 days to CSW (Fig. 5a) or Cm-treated CSW (not shown) showed no regression of CEA. Additionally, Cm did not have an inhibitory effect on CEA regression, as evidenced by com- plete regression of CEA from squid infected with the Cm- resistant strain ESI 14-U2 and treated with Cm (Fig. 5c).. hrs exposed Figure 6. Relative percentage of light organs in 4-day-old squid showing stage 3 regression of the CEA (see text). Hatchling squid were exposed to ESI 14 for 1. 4. 8, and 12 hours, followed by treatment with Cm (see Fig. 3B-for experimental design). Values are reported as E/C. the percentage of Cm-treated squid (experimental) showing regression divided by the percentage of infected controls showing regression. Each point represents values pooled from four separate experiments (see Table I). Vertical bars represent the full range of data for the four experiments. Variable transient exposure to V. fischeri strain ESI 14 Exposure of squid to bacteria for 1, 4, 8, 12, or 14 hours to determine the minimum time requirement for induc- tion of CEA regression revealed that only those squid ex- posed to bacteria for at least 12 h showed CEA regression (E/C = ) comparable to that of continuously exposed control squid (Fig. 6: Table I). These squid were visibly luminous at the time of Cm treatment. Squid exposed to bacteria for 1 or 4 h were not luminous and showed no CEA regression. Those individuals expos
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
